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Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 EMBO+J 2015 ; 34 (22): 2758-74 Nephropedia Template TP
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Alu element?containing RNAs maintain nucleolar structure and function #MMPMID26464461
Caudron?Herger M; Pankert T; Seiler J; Németh A; Voit R; Grummt I; Rippe K
EMBO J 2015[Nov]; 34 (22): 2758-74 PMID26464461show ga
Non?coding RNAs play a key role in organizing the nucleus into functional subcompartments. By combining fluorescence microscopy and RNA deep?sequencing?based analysis, we found that RNA polymerase II transcripts originating from intronic Alu elements (aluRNAs) were enriched in the nucleolus. Antisense?oligo?mediated depletion of aluRNAs or drug?induced inhibition of RNA polymerase II activity disrupted nucleolar structure and impaired RNA polymerase I?dependent transcription of rRNA genes. In contrast, overexpression of a prototypic aluRNA sequence increased both nucleolus size and levels of pre?rRNA, suggesting a functional link between aluRNA, nucleolus integrity and pre?rRNA synthesis. Furthermore, we show that aluRNAs interact with nucleolin and target ectopic genomic loci to the nucleolus. Our study suggests an aluRNA?based mechanism that links RNA polymerase I and II activities and modulates nucleolar structure and rRNA production.