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10.1038/srep18311 http://scihub22266oqcxt.onion/10.1038/srep18311 C4682130!4682130 !26674602     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26674602 &cmd=llinks): Failed to open stream: HTTP request failed! 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Autophagic degradation of aquaporin-2 is an early event in hypokalemia-induced nephrogenic diabetes insipidus |pdf=|usr=}}{{26674602 }} gab.com Text Autophagic degradation of aquaporin-2 is an early event in hypokalemia-induced nephrogenic diabetes insipidus JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26674602 #FOAvip Hypokalemia (low serum potassium level) is a common electrolyte imbalance that can cause a defect in urinary concentrating ability, i.e., nephrogenic diabetes insipidus (NDI), but the molecular mechanism is unknown. We employed proteomic analysis of inner medullary collecting ducts (IMCD) from rats fed with a potassium-free diet for 1 day. IMCD protein quantification was performed by mass spectrometry using a label-free methodology. A total of 131 proteins, including the water channel AQP2, exhibited significant changes in abundance, most of which were decreased. Bioinformatic analysis revealed that many of the down-regulated proteins were associated with the biological processes of generation of precursor metabolites and energy, actin cytoskeleton organization, and cell-cell adhesion. Targeted LC-MS/MS and immunoblotting studies further confirmed the down regulation of 18 selected proteins. Electron microscopy showed autophagosomes/autophagolysosomes in the IMCD cells of rats deprived of potassium for only 1 day. An increased number of autophagosomes was also confirmed by immunofluorescence, demonstrating co-localization of LC3 and Lamp1 with AQP2 and several other down-regulated proteins in IMCD cells. AQP2 was also detected in autophagosomes in IMCD cells of potassium-deprived rats by immunogold electron microscopy. Thus, enhanced autophagic degradation of proteins, most notably including AQP2, is an early event in hypokalemia-induced NDI. Twit Text FOAVip Autophagic degradation of aquaporin-2 is an early event in hypokalemia-induced nephrogenic diabetes insipidus ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26674602 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26674602 #FOAvip English Wikipedia <ref>{{Cite pmid|26674602 }}</ref> Autophagic degradation of aquaporin-2 is an early event in hypokalemia-induced nephrogenic diabetes insipidus #MMPMID26674602 Khositseth S ; Uawithya P ; Somparn P ; Charngkaew K ; Thippamom N ; Hoffert JD ; Saeed F ; Michael Payne D ; Chen SH ; Fenton RA ; Pisitkun T Sci Rep 2015[Dec]; 5 (ä): 18311 PMID26674602 show ga Hypokalemia (low serum potassium level) is a common electrolyte imbalance that can cause a defect in urinary concentrating ability, i.e., nephrogenic diabetes insipidus (NDI), but the molecular mechanism is unknown. We employed proteomic analysis of inner medullary collecting ducts (IMCD) from rats fed with a potassium-free diet for 1 day. IMCD protein quantification was performed by mass spectrometry using a label-free methodology. A total of 131 proteins, including the water channel AQP2, exhibited significant changes in abundance, most of which were decreased. Bioinformatic analysis revealed that many of the down-regulated proteins were associated with the biological processes of generation of precursor metabolites and energy, actin cytoskeleton organization, and cell-cell adhesion. Targeted LC-MS/MS and immunoblotting studies further confirmed the down regulation of 18 selected proteins. Electron microscopy showed autophagosomes/autophagolysosomes in the IMCD cells of rats deprived of potassium for only 1 day. An increased number of autophagosomes was also confirmed by immunofluorescence, demonstrating co-localization of LC3 and Lamp1 with AQP2 and several other down-regulated proteins in IMCD cells. AQP2 was also detected in autophagosomes in IMCD cells of potassium-deprived rats by immunogold electron microscopy. Thus, enhanced autophagic degradation of proteins, most notably including AQP2, is an early event in hypokalemia-induced NDI. |*Autophagy [MESH] |Actin Cytoskeleton/metabolism [MESH] |Animals [MESH] |Aquaporin 2/*metabolism [MESH] |Chromatography, Liquid [MESH] |Diabetes Insipidus, Nephrogenic/*metabolism/physiopathology [MESH] |Hypokalemia/*metabolism/physiopathology [MESH] |Immunoblotting [MESH] |Kidney Medulla/metabolism [MESH] |Kidney Tubules, Collecting/metabolism/ultrastructure [MESH] |Lysosomal Membrane Proteins/metabolism [MESH] |Male [MESH] |Microscopy, Immunoelectron [MESH] |Microtubule-Associated Proteins/metabolism [MESH] |Phagosomes/metabolism/ultrastructure [MESH] |Proteome/metabolism [MESH] |Proteomics/methods [MESH] |Rats, Sprague-Dawley [MESH] |Tandem Mass Spectrometry [MESH]Autophagic degradation of aquaporin-2 is an early event in hypokalemia(epmc).pdf DeepDyve Pubget Overpricing