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10.1038/srep18312 http://scihub22266oqcxt.onion/10.1038/srep18312 C4682128!4682128!26674741     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2015 ; 5 (ä): ä Nephropedia Template TP {{tp|p=26674741|t=2015. TALEN-mediated homologous recombination in Daphnia magna |pdf=|usr=}}{{26674741}} gab.com Text TALEN-mediated homologous recombination in Daphnia magna JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26674741 #FOAvip Transcription Activator-Like Effector Nucleases (TALENs) offer versatile tools to engineer endogenous genomic loci in various organisms. We established a homologous recombination (HR)-based knock-in using TALEN in the crustacean Daphnia magna, a model for ecological and toxicological genomics. We constructed TALENs and designed the 67?bp donor insert targeting a point deletion in the eyeless mutant that shows eye deformities. Co-injection of the TALEN mRNA with donor DNA into eggs led to the precise integration of the donor insert in the germ line, which recovered eye deformities in offspring. The frequency of HR events in the germ line was 2% by using both plasmid and single strand oligo DNA with 1.5?kb and 80?nt homology to the target. Deficiency of ligase 4 involved in non-homologous end joining repair did not increase the HR efficiency. Our data represent efficient HR-based knock-in by TALENs in D. magna, which is a promising tool to understand Daphnia gene functions. Twit Text FOAVip TALEN-mediated homologous recombination in Daphnia magna ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26674741 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26674741 #FOAvip English Wikipedia <ref>{{Cite pmid|26674741}}</ref> TALEN-mediated homologous recombination in Daphnia magna #MMPMID26674741 Nakanishi T; Kato Y; Matsuura T; Watanabe H Sci Rep 2015[]; 5 (ä): ä PMID26674741show ga Transcription Activator-Like Effector Nucleases (TALENs) offer versatile tools to engineer endogenous genomic loci in various organisms. We established a homologous recombination (HR)-based knock-in using TALEN in the crustacean Daphnia magna, a model for ecological and toxicological genomics. We constructed TALENs and designed the 67?bp donor insert targeting a point deletion in the eyeless mutant that shows eye deformities. Co-injection of the TALEN mRNA with donor DNA into eggs led to the precise integration of the donor insert in the germ line, which recovered eye deformities in offspring. The frequency of HR events in the germ line was 2% by using both plasmid and single strand oligo DNA with 1.5?kb and 80?nt homology to the target. Deficiency of ligase 4 involved in non-homologous end joining repair did not increase the HR efficiency. Our data represent efficient HR-based knock-in by TALENs in D. magna, which is a promising tool to understand Daphnia gene functions. äTALEN-mediated homologous recombination in Daphnia magna (epmc).pdf DeepDyve Pubget Overpricing