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suck abstract from ncbi


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pmid26722509
      Int+J+Clin+Exp+Pathol 2015 ; 8 (10 ): 13114-9
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  • Sevoflurane pretreatment enhance HIF-2? expression in mice after renal ischemia/reperfusion injury #MMPMID26722509
  • Zheng B ; Zhan Q ; Chen J ; Xu H ; He Z
  • Int J Clin Exp Pathol 2015[]; 8 (10 ): 13114-9 PMID26722509 show ga
  • Ischemia/reperfusion (I/R) injury often occurs, which is one of the major causes of acute kidney injury, thus increasing in-hospital mortality. HIF-2? has a protective role against ischemia of the kidney. Renal ischemia/reperfusion under sevoflurane anesthesia resulted in drastic improvements in renal function. We hypothesized that underlying mechanism responsible for renal protection from sevoflurane pretreatment involves the upregulation of HIF-2?. Sevoflurane pretreatment were performed on WT and HIF-2? knockout mice before renal ischemia/reperfusion. Levels of blood urea nitrogen (BUN) and serum creatinine (Cr) were determined with a standard clinical automatic analyzer. The left kidneys were taken for morphological examination. Expression of HIF-2? in kidney tissue was examined by western blotting. In WT mice, group I/R injury had significantly higher BUN and Cr levels than group control, whereas group I/R + Sev had significantly lower BUN and Cr levels than group I/R injury. Renal HIF-2? expression levels were significantly higher in WT mice of group I/R + Sev than group control and group I/R. In HIF-2?(-/-) mice, group I/R + Sev showed much higher BUN and Cr levels and severer histological damage than group I/R and group control. Renal HIF-2? expression levels were significantly higher in WT mice of group I/R + Sev than group control and group I/R. Our findings suggested that HIF-2? might contribute to the beneficial effect of sevoflurane in renal ischemia/reperfusion injury.
  • |Acute Kidney Injury/metabolism/*physiopathology [MESH]
  • |Animals [MESH]
  • |Anti-Inflammatory Agents/*pharmacology [MESH]
  • |Basic Helix-Loop-Helix Transcription Factors/*biosynthesis [MESH]
  • |Blotting, Western [MESH]
  • |Disease Models, Animal [MESH]
  • |Kidney Function Tests [MESH]
  • |Methyl Ethers/*pharmacology [MESH]
  • |Mice [MESH]
  • |Mice, Knockout [MESH]
  • |Reperfusion Injury/metabolism/*physiopathology [MESH]
  • |Sevoflurane [MESH]


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