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10.1038/nmeth.3624 http://scihub22266oqcxt.onion/10.1038/nmeth.3624 C4679368!4679368!26480473     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Methods 2015 ; 12 (12): 1150-6 Nephropedia Template TP {{tp|p=26480473|t=2015. DNA-binding domain fusions enhance the targeting range and precision of Cas9 |pdf=|usr=}}{{26480473}} gab.com Text DNA-binding domain fusions enhance the targeting range and precision of Cas9 JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=26480473 #FOAvip The CRISPR-Cas9 system is commonly employed in biomedical research; however, the precision of Cas9 is sub-optimal for gene therapy applications that involve editing a large population of cells. Variations on the standard Cas9 system have yielded improvements in the precision of targeted DNA cleavage, but often restrict the range of targetable sequences. It remains unclear whether these variants can limit lesions to a single site within the human genome over a large cohort of treated cells. Here, we demonstrate that fusing a programmable DNA-binding domain (pDBD) to Cas9 combined with the attenuation of Cas9?s inherent DNA binding affinity produces a Cas9-pDBD chimera with dramatically improved precision and increased targeting range. Because the specificity and affinity of this framework is easily tuned, Cas9-pDBDs provide a flexible system that can be tailored to achieve extremely precise genome editing at nearly any genomic locus ? characteristics that are ideal for gene therapy applications. Twit Text FOAVip DNA-binding domain fusions enhance the targeting range and precision of Cas9 ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=26480473 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26480473 #FOAvip English Wikipedia <ref>{{Cite pmid|26480473}}</ref> DNA-binding domain fusions enhance the targeting range and precision of Cas9 #MMPMID26480473 Bolukbasi MF; Gupta A; Oikemus S; Derr AG; Garber M; Brodsky MH; Zhu LJ; Wolfe SA Nat Methods 2015[Dec]; 12 (12): 1150-6 PMID26480473show ga The CRISPR-Cas9 system is commonly employed in biomedical research; however, the precision of Cas9 is sub-optimal for gene therapy applications that involve editing a large population of cells. Variations on the standard Cas9 system have yielded improvements in the precision of targeted DNA cleavage, but often restrict the range of targetable sequences. It remains unclear whether these variants can limit lesions to a single site within the human genome over a large cohort of treated cells. Here, we demonstrate that fusing a programmable DNA-binding domain (pDBD) to Cas9 combined with the attenuation of Cas9?s inherent DNA binding affinity produces a Cas9-pDBD chimera with dramatically improved precision and increased targeting range. Because the specificity and affinity of this framework is easily tuned, Cas9-pDBDs provide a flexible system that can be tailored to achieve extremely precise genome editing at nearly any genomic locus ? characteristics that are ideal for gene therapy applications. äDNA-binding domain fusions enhance the targeting range and precision o(epmc).pdf DeepDyve Pubget Overpricing