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10.1364/BOE.6.004694

http://scihub22266oqcxt.onion/10.1364/BOE.6.004694
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C4679247!4679247!26713187
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suck abstract from ncbi


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pmid26713187      Biomed+Opt+Express 2015 ; 6 (12): 4694-704
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  • Selective disruption of vascular endothelium of zebrafish embryos by ultrafast laser microsurgical treatment #MMPMID26713187
  • Woo SY; Moon HY; Kim TG; Lee HS; Sidhu MS; Kim C; Jeon JP; Jeoung SC
  • Biomed Opt Express 2015[Dec]; 6 (12): 4694-704 PMID26713187show ga
  • In this work, we demonstrate that ultrafast laser irradiation could selectively disrupt vascular endothelium of zebrafish embryos in vivo. Ultrafast lasers minimize the collateral damage in the vicinity of the laser focus and eventually reduce coagulation in the tissues. We have also found that the threshold fluence for lesion formation of the vascular endothelium strongly depends on the developmental stage of the embryos. The threshold laser fluence required to induce apparent lesions in the vascular structure for Somite 14, 20 and 25 stages is about 5 J/cm2 ~7 J/cm2, which is much lower than that for the later development stages of Prim 16 and Prim 20 of 30 J/cm2 ~50 J/cm2. The proposed method for treating the vascular cord of zebrafish embryos in the early stage of development has potential as a selective and effective method to induce a fatal lesion in the vascular endothelium without damaging the developed blood vessels.
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