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10.1073/pnas.1520957112 http://scihub22266oqcxt.onion/10.1073/pnas.1520957112 C4679030!4679030 !26598700     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26598700 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Proc+Natl+Acad+Sci+U+S+A 2015 ; 112 (49 ): E6752-61 Nephropedia Template TP {{tp|p=26598700 |t=2015. ER trapping reveals Golgi enzymes continually revisit the ER through a recycling pathway that controls Golgi organization |pdf=|usr=}}{{26598700 }} gab.com Text ER trapping reveals Golgi enzymes continually revisit the ER through a recycling pathway that controls Golgi organization JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=26598700 #FOAvip Whether Golgi enzymes remain localized within the Golgi or constitutively cycle through the endoplasmic reticulum (ER) is unclear, yet is important for understanding Golgi dependence on the ER. Here, we demonstrate that the previously reported inefficient ER trapping of Golgi enzymes in a rapamycin-based assay results from an artifact involving an endogenous ER-localized 13-kD FK506 binding protein (FKBP13) competing with the FKBP12-tagged Golgi enzyme for binding to an FKBP-rapamycin binding domain (FRB)-tagged ER trap. When we express an FKBP12-tagged ER trap and FRB-tagged Golgi enzymes, conditions precluding such competition, the Golgi enzymes completely redistribute to the ER upon rapamycin treatment. A photoactivatable FRB-Golgi enzyme, highlighted only in the Golgi, likewise redistributes to the ER. These data establish Golgi enzymes constitutively cycle through the ER. Using our trapping scheme, we identify roles of rab6a and calcium-independent phospholipase A2 (iPLA2) in Golgi enzyme recycling, and show that retrograde transport of Golgi membrane underlies Golgi dispersal during microtubule depolymerization and mitosis. Twit Text FOAVip ER trapping reveals Golgi enzymes continually revisit the ER through a recycling pathway that controls Golgi organization ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=26598700 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26598700 #FOAvip English Wikipedia <ref>{{Cite pmid|26598700 }}</ref> ER trapping reveals Golgi enzymes continually revisit the ER through a recycling pathway that controls Golgi organization #MMPMID26598700 Sengupta P ; Satpute-Krishnan P ; Seo AY ; Burnette DT ; Patterson GH ; Lippincott-Schwartz J Proc Natl Acad Sci U S A 2015[Dec]; 112 (49 ): E6752-61 PMID26598700 show ga Whether Golgi enzymes remain localized within the Golgi or constitutively cycle through the endoplasmic reticulum (ER) is unclear, yet is important for understanding Golgi dependence on the ER. Here, we demonstrate that the previously reported inefficient ER trapping of Golgi enzymes in a rapamycin-based assay results from an artifact involving an endogenous ER-localized 13-kD FK506 binding protein (FKBP13) competing with the FKBP12-tagged Golgi enzyme for binding to an FKBP-rapamycin binding domain (FRB)-tagged ER trap. When we express an FKBP12-tagged ER trap and FRB-tagged Golgi enzymes, conditions precluding such competition, the Golgi enzymes completely redistribute to the ER upon rapamycin treatment. A photoactivatable FRB-Golgi enzyme, highlighted only in the Golgi, likewise redistributes to the ER. These data establish Golgi enzymes constitutively cycle through the ER. Using our trapping scheme, we identify roles of rab6a and calcium-independent phospholipase A2 (iPLA2) in Golgi enzyme recycling, and show that retrograde transport of Golgi membrane underlies Golgi dispersal during microtubule depolymerization and mitosis. |Animals [MESH] |COS Cells [MESH] |Chlorocebus aethiops [MESH] |Endoplasmic Reticulum/*metabolism [MESH] |Golgi Apparatus/*enzymology [MESH] |HeLa Cells [MESH] |Humans [MESH] |Mitosis [MESH] |Phospholipases A2, Calcium-Independent/physiology [MESH] |Sirolimus/pharmacology [MESH] |Tacrolimus Binding Protein 1A/metabolism [MESH] |Tacrolimus Binding Proteins/metabolism [MESH]ER trapping reveals Golgi enzymes continually revisit the ER through a(epmc).pdf DeepDyve Pubget Overpricing