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10.1016/j.biomaterials.2014.01.024 http://scihub22266oqcxt.onion/10.1016/j.biomaterials.2014.01.024 C4678102!4678102 !24462361     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24462361 &cmd=llinks): Failed to open stream: HTTP request failed! 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Cryo-chemical decellularization of the whole liver for mesenchymal stem cells-based functional hepatic tissue engineering |pdf=|usr=}}{{24462361 }} gab.com Text Cryo-chemical decellularization of the whole liver for mesenchymal stem cells-based functional hepatic tissue engineering JO: Biomaterials http://www.kidney.de/pget.php?&tw=1&pmid=24462361 #FOAvip Liver transplantation is the ultimate treatment for severe hepatic failure to date. However, the limited supply of donor organs has severely hampered this treatment. So far, great potentials of using mesenchymal stem cells (MSCs) to replenish the hepatic cell population have been shown; nevertheless, there still is a lack of an optimal three-dimensional scaffold for generation of well-transplantable hepatic tissues. In this study, we utilized a cryo-chemical decellularization method which combines physical and chemical approach to generate acellular liver scaffolds (ALS) from the whole liver. The produced ALS provides a biomimetic three-dimensional environment to support hepatic differentiation of MSCs, evidenced by expression of hepatic-associated genes and marker protein, glycogen storage, albumin secretion, and urea production. It is also found that hepatic differentiation of MSCs within the ALS is much more efficient than two-dimensional culture in vitro. Importantly, the hepatic-like tissues (HLT) generated by repopulating ALS with MSCs are able to act as functional grafts and rescue lethal hepatic failure after transplantation in vivo. In summary, the cryo-chemical method used in this study is suitable for decellularization of liver and create acellular scaffolds that can support hepatic differentiation of MSCs and be used to fabricate functional tissue-engineered liver constructs. Twit Text FOAVip Cryo-chemical decellularization of the whole liver for mesenchymal stem cells-based functional hepatic tissue engineering ????Biomaterials http://www.kidney.de/pget.php?&tw=1&pmid=24462361 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24462361 #FOAvip English Wikipedia <ref>{{Cite pmid|24462361 }}</ref> Cryo-chemical decellularization of the whole liver for mesenchymal stem cells-based functional hepatic tissue engineering #MMPMID24462361 Jiang WC ; Cheng YH ; Yen MH ; Chang Y ; Yang VW ; Lee OK Biomaterials 2014[Apr]; 35 (11 ): 3607-17 PMID24462361 show ga Liver transplantation is the ultimate treatment for severe hepatic failure to date. However, the limited supply of donor organs has severely hampered this treatment. So far, great potentials of using mesenchymal stem cells (MSCs) to replenish the hepatic cell population have been shown; nevertheless, there still is a lack of an optimal three-dimensional scaffold for generation of well-transplantable hepatic tissues. In this study, we utilized a cryo-chemical decellularization method which combines physical and chemical approach to generate acellular liver scaffolds (ALS) from the whole liver. The produced ALS provides a biomimetic three-dimensional environment to support hepatic differentiation of MSCs, evidenced by expression of hepatic-associated genes and marker protein, glycogen storage, albumin secretion, and urea production. It is also found that hepatic differentiation of MSCs within the ALS is much more efficient than two-dimensional culture in vitro. Importantly, the hepatic-like tissues (HLT) generated by repopulating ALS with MSCs are able to act as functional grafts and rescue lethal hepatic failure after transplantation in vivo. In summary, the cryo-chemical method used in this study is suitable for decellularization of liver and create acellular scaffolds that can support hepatic differentiation of MSCs and be used to fabricate functional tissue-engineered liver constructs. |*Cold Temperature [MESH] |Animals [MESH] |Biomarkers/metabolism [MESH] |Cell Differentiation/genetics [MESH] |Gene Expression Regulation [MESH] |Liver Transplantation [MESH] |Liver/metabolism/*pathology/physiopathology/ultrastructure [MESH] |Mesenchymal Stem Cells/*cytology/metabolism [MESH] |Mice [MESH] |Mice, Inbred BALB C [MESH] |Mice, SCID [MESH] |Real-Time Polymerase Chain Reaction [MESH] |Tissue Engineering/*methods [MESH]Cryo-chemical decellularization of the whole liver for mesenchymal ste(epmc).pdf DeepDyve Pubget Overpricing