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Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Sci+Rep 2015 ; 5 (ä): ä Nephropedia Template TP
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Zepto-molar electrochemical detection of Brucella genome based on gold nanoribbons covered by gold nanoblooms #MMPMID26657828
Rahi A; Sattarahmady N; Heli H
Sci Rep 2015[]; 5 (ä): ä PMID26657828show ga
Gold nanoribbons covered by gold nanoblooms were sonoelectrodeposited on a polycrystalline gold surface at ?1800?mV (vs. AgCl) with the assistance of ultrasound and co-occurrence of the hydrogen evolution reaction. The nanostructure, as a transducer, was utilized to immobilize a Brucella-specific probe and fabrication of a genosensor, and the process of immobilization and hybridization was detected by electrochemical methods, using methylene blue as a redox marker. The proposed method for detection of the complementary sequence, sequences with base-mismatched (one-, two- and three-base mismatches), and the sequence of non-complementary sequence was assayed. The fabricated genosensor was evaluated for the assay of the bacteria in the cultured and human samples without polymerase chain reactions (PCR). The genosensor could detect the complementary sequence with a calibration sensitivity of 0.40??A dm3 mol?1, a linear concentration range of 10?zmol dm?3 to 10?pmol dm?3, and a detection limit of 1.71?zmol dm?3.