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10.1371/journal.pcbi.1004634

http://scihub22266oqcxt.onion/10.1371/journal.pcbi.1004634
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suck abstract from ncbi


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pmid26657340      PLoS+Comput+Biol 2015 ; 11 (12): ä
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  • MIiSR: Molecular Interactions in Super-Resolution Imaging Enables the Analysis of Protein Interactions, Dynamics and Formation of Multi-protein Structures #MMPMID26657340
  • Caetano FA; Dirk BS; Tam JHK; Cavanagh PC; Goiko M; Ferguson SSG; Pasternak SH; Dikeakos JD; de Bruyn JR; Heit B
  • PLoS Comput Biol 2015[Dec]; 11 (12): ä PMID26657340show ga
  • Our current understanding of the molecular mechanisms which regulate cellular processes such as vesicular trafficking has been enabled by conventional biochemical and microscopy techniques. However, these methods often obscure the heterogeneity of the cellular environment, thus precluding a quantitative assessment of the molecular interactions regulating these processes. Herein, we present Molecular Interactions in Super Resolution (MIiSR) software which provides quantitative analysis tools for use with super-resolution images. MIiSR combines multiple tools for analyzing intermolecular interactions, molecular clustering and image segmentation. These tools enable quantification, in the native environment of the cell, of molecular interactions and the formation of higher-order molecular complexes. The capabilities and limitations of these analytical tools are demonstrated using both modeled data and examples derived from the vesicular trafficking system, thereby providing an established and validated experimental workflow capable of quantitatively assessing molecular interactions and molecular complex formation within the heterogeneous environment of the cell.
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