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10.1097/PRS.0000000000001446 http://scihub22266oqcxt.onion/10.1097/PRS.0000000000001446 C4676560!4676560!26218393     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Plast+Reconstr+Surg 2015 ; 136 (2): 189e-99e Nephropedia Template TP {{tp|p=26218393|t=2015. Differential effects of processing time and duration of collagenase digestion on human and murine fat grafts |pdf=|usr=}}{{26218393}} gab.com Text Differential effects of processing time and duration of collagenase digestion on human and murine fat grafts JO: Plast Reconstr Surg http://www.kidney.de/pget.php?&tw=1&pmid=26218393 #FOAvip Background: Autologous fat graft retention is unpredictable and mechanisms of optimization are poorly understood. Attempts at improving retention utilize collagenase experimentally and clinically to isolate the stromal vascular fraction to ?enhance? fat grafts. However, no standardized duration for collagenase digestion or time following fat graft harvest has been established. This study investigates the effect of 1.) time after fat graft harvest and 2.) collagenase digestion time on interstitial cell and adipocyte viability in murine fat and human lipoaspirate. Methods: Murine fat and human lipoaspirate were incubated ex vivo after harvest at room temperature for 120 minutes. Additional groups were incubated with collagenase for increasing five minute intervals from 30-60 minutes. Samples from each group were stained with BODIPY to quantify intact adipocytes and LIVE/DEAD kit to quantify interstitial cell viability. Results: With increased time post-harvest, the number of intact adipocytes in murine fat and human lipoaspirate remained unchanged. Human interstitial cells were resistant to the effect of increased time ex vivo, while murine interstitial cells decreased in viability. In both populations, increased collagenase digestion time significantly decreased the number of viable adipocytes (murine: p-value ? 0.001, human: p-value ? 0.001) and interstitial cells (murine: p-value ? 0.001, human: p-value ? 0.001). Conclusions: Human and murine adipocytes and human interstitial cells appear resistant to deleterious effects of increasing time following harvest. However, murine interstitial cells including are sensitive to increased time and prolonged collagenase digestion. These studies highlight the complex cellular components of fat grafts and how they respond differentially to time and collagenase digestion. Twit Text FOAVip Differential effects of processing time and duration of collagenase digestion on human and murine fat grafts ????Plast Reconstr Surg http://www.kidney.de/pget.php?&tw=1&pmid=26218393 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26218393 #FOAvip English Wikipedia <ref>{{Cite pmid|26218393}}</ref> Differential effects of processing time and duration of collagenase digestion on human and murine fat grafts #MMPMID26218393 Seaman S; Tannan S; Cao Y; Peirce S; Lin K Plast Reconstr Surg 2015[Aug]; 136 (2): 189e-99e PMID26218393show ga Background: Autologous fat graft retention is unpredictable and mechanisms of optimization are poorly understood. Attempts at improving retention utilize collagenase experimentally and clinically to isolate the stromal vascular fraction to ?enhance? fat grafts. However, no standardized duration for collagenase digestion or time following fat graft harvest has been established. This study investigates the effect of 1.) time after fat graft harvest and 2.) collagenase digestion time on interstitial cell and adipocyte viability in murine fat and human lipoaspirate. Methods: Murine fat and human lipoaspirate were incubated ex vivo after harvest at room temperature for 120 minutes. Additional groups were incubated with collagenase for increasing five minute intervals from 30-60 minutes. Samples from each group were stained with BODIPY to quantify intact adipocytes and LIVE/DEAD kit to quantify interstitial cell viability. Results: With increased time post-harvest, the number of intact adipocytes in murine fat and human lipoaspirate remained unchanged. Human interstitial cells were resistant to the effect of increased time ex vivo, while murine interstitial cells decreased in viability. In both populations, increased collagenase digestion time significantly decreased the number of viable adipocytes (murine: p-value ? 0.001, human: p-value ? 0.001) and interstitial cells (murine: p-value ? 0.001, human: p-value ? 0.001). Conclusions: Human and murine adipocytes and human interstitial cells appear resistant to deleterious effects of increasing time following harvest. However, murine interstitial cells including are sensitive to increased time and prolonged collagenase digestion. These studies highlight the complex cellular components of fat grafts and how they respond differentially to time and collagenase digestion. äDifferential effects of processing time and duration of collagenase di(epmc).pdf DeepDyve Pubget Overpricing