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2015 ; 5
(ä): 17975
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Only a minority of broad-range detoxification genes respond to a variety of
phytotoxins in generalist Bemisia tabaci species
#MMPMID26655836
Halon E
; Eakteiman G
; Moshitzky P
; Elbaz M
; Alon M
; Pavlidi N
; Vontas J
; Morin S
Sci Rep
2015[Dec]; 5
(ä): 17975
PMID26655836
show ga
Generalist insect can utilize two different modes for regulating their
detoxification genes, the constitutive mode and the induced mode. Here, we used
the Bemisia tabaci sibling species MEAM1 and MED, as a model system for studying
constitutive and induced detoxification resistance and their associated
tradeoffs. B. tabaci adults were allowed to feed through membranes for 24?h on
diet containing only sucrose or sucrose with various phytotoxins. Quantitative
real-time PCR analyses of 18 detoxification genes, indicated that relatively few
transcripts were changed in both the MEAM1 and MED species, in response to the
addition of phytotoxins to the diet. Induced transcription of detoxification
genes only in the MED species, in response to the presence of indole-3-carbinol
in the insect's diet, was correlated with maintenance of reproductive performance
in comparison to significant reduction in performance of the MEAM1 species. Three
genes, COE2, CYP6-like 5 and BtGST2, responded to more than one compound and were
highly transcribed in the insect gut. Furthermore, functional assays showed that
the BtGST2 gene encodes a protein capable of interacting with both flavonoids and
glucosinolates. In conclusion, several detoxification genes were identified that
could potentially be involved in the adaptation of B. tabaci to its host plants.