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2015 ; 211
(5
): 963-73
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English Wikipedia
The polarity protein Inturned links NPHP4 to Daam1 to control the subapical actin
network in multiciliated cells
#MMPMID26644512
Yasunaga T
; Hoff S
; Schell C
; Helmstädter M
; Kretz O
; Kuechlin S
; Yakulov TA
; Engel C
; Müller B
; Bensch R
; Ronneberger O
; Huber TB
; Lienkamp SS
; Walz G
J Cell Biol
2015[Dec]; 211
(5
): 963-73
PMID26644512
show ga
Motile cilia polarization requires intracellular anchorage to the cytoskeleton;
however, the molecular machinery that supports this process remains elusive. We
report that Inturned plays a central role in coordinating the interaction between
cilia-associated proteins and actin-nucleation factors. We observed that
knockdown of nphp4 in multiciliated cells of the Xenopus laevis epidermis
compromised ciliogenesis and directional fluid flow. Depletion of nphp4 disrupted
the subapical actin layer. Comparison to the structural defects caused by
inturned depletion revealed striking similarities. Furthermore,
coimmunoprecipitation assays demonstrated that the two proteins interact with
each other and that Inturned mediates the formation of ternary protein complexes
between NPHP4 and DAAM1. Knockdown of daam1, but not formin-2, resulted in
similar disruption of the subapical actin web, whereas nphp4 depletion prevented
the association of Inturned with the basal bodies. Thus, Inturned appears to
function as an adaptor protein that couples cilia-associated molecules to
actin-modifying proteins to rearrange the local actin cytoskeleton.
|Actin Cytoskeleton/metabolism
[MESH]
|Actins/*metabolism
[MESH]
|Adaptor Proteins, Signal Transducing/*metabolism
[MESH]
free
free
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