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10.14814/phy2.12611 http://scihub22266oqcxt.onion/10.14814/phy2.12611 C4673640!4673640!26564065     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Physiol+Rep 2015 ; 3 (11): ä Nephropedia Template TP {{tp|p=26564065|t=2015. Effects of urine composition on epithelial Na+ channel-targeted protease activity |pdf=|usr=}}{{26564065}} gab.com Text Effects of urine composition on epithelial Na+ channel-targeted protease activity JO: Physiol Rep http://www.kidney.de/pget.php?&tw=1&pmid=26564065 #FOAvip We examined human urinary proteolytic activity toward the Epithelial Sodium Channel (ENaC). We focused on two sites in each of alpha and gamma ENaC that are targets of endogenous and exogenous proteases. We examined the effects of ionic strength, pH and urinary H+-buffers, metabolic intermediates, redox molecules, and large urinary proteins. Monoatomic cations caused the largest effect, with sodium inhibiting activity in the 15?515 mEq range. Multivalent cations zinc and copper inhibited urinary proteolytic activity at concentrations below 100 ?mol/L. Similar to sodium, urea caused a 30% inhibition in the 0?500 mmol/L range. This was not observed with acetone and ethanol. Modulating urinary redox status modified activity with H2O2 stimulated and ascorbate inhibited activity. Minimal effects (<10%) were observed with caffeine, glucose, several TCA cycle intermediates, salicylic acid, inorganic phosphate, albumin, creatinine, and Tamm?Horsfall protein. The cumulative activity of ENaC-cleaving proteases was highest at neutral pH, however, alpha and gamma proteases exhibited an inverse dependence with alpha stimulated at acidic and gamma stimulated at alkaline pH. These data indicate that ENaC-targeting urinary proteolytic activity is sensitive to sodium, urea and pH and changes in these components can modify channel cleavage and activation status, and likely downstream sodium absorption unrelated to changes in protein or channel density. Twit Text FOAVip Effects of urine composition on epithelial Na+ channel-targeted protease activity ????Physiol Rep http://www.kidney.de/pget.php?&tw=1&pmid=26564065 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26564065 #FOAvip English Wikipedia <ref>{{Cite pmid|26564065}}</ref> Effects of urine composition on epithelial Na+ channel-targeted protease activity #MMPMID26564065 Berman JM; Awayda RG; Awayda MS Physiol Rep 2015[Nov]; 3 (11): ä PMID26564065show ga We examined human urinary proteolytic activity toward the Epithelial Sodium Channel (ENaC). We focused on two sites in each of alpha and gamma ENaC that are targets of endogenous and exogenous proteases. We examined the effects of ionic strength, pH and urinary H+-buffers, metabolic intermediates, redox molecules, and large urinary proteins. Monoatomic cations caused the largest effect, with sodium inhibiting activity in the 15?515 mEq range. Multivalent cations zinc and copper inhibited urinary proteolytic activity at concentrations below 100 ?mol/L. Similar to sodium, urea caused a 30% inhibition in the 0?500 mmol/L range. This was not observed with acetone and ethanol. Modulating urinary redox status modified activity with H2O2 stimulated and ascorbate inhibited activity. Minimal effects (<10%) were observed with caffeine, glucose, several TCA cycle intermediates, salicylic acid, inorganic phosphate, albumin, creatinine, and Tamm?Horsfall protein. The cumulative activity of ENaC-cleaving proteases was highest at neutral pH, however, alpha and gamma proteases exhibited an inverse dependence with alpha stimulated at acidic and gamma stimulated at alkaline pH. These data indicate that ENaC-targeting urinary proteolytic activity is sensitive to sodium, urea and pH and changes in these components can modify channel cleavage and activation status, and likely downstream sodium absorption unrelated to changes in protein or channel density. äEffects of urine composition on epithelial Na+ channel-targeted protea(epmc).pdf DeepDyve Pubget Overpricing