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10.3109/10409238.2015.1023252

http://scihub22266oqcxt.onion/10.3109/10409238.2015.1023252
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C4673598!4673598!25788028
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suck abstract from ncbi


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pmid25788028      Crit+Rev+Biochem+Mol+Biol 2015 ; 50 (3): 231-41
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  • Towards reconstitution of membrane fusion mediated by SNAREs and other synaptic proteins #MMPMID25788028
  • Brunger AT; Cipriano DJ; Diao J
  • Crit Rev Biochem Mol Biol 2015[May]; 50 (3): 231-41 PMID25788028show ga
  • Proteoliposomes have been widely used for in vitro studies of membrane fusion mediated by synaptic proteins. Initially, such studies were made with large unsynchronized ensembles of vesicles. Such ensemble assays limited the insights into the SNARE-mediated fusion mechanism that could be obtained from them. Single particle microscopy experiments can alleviate many of these limitations but they pose significant technical challenges. Here we summarize various approaches that have enabled studies of fusion mediated by SNAREs and other synaptic proteins at a single-particle level. Currently available methods are described and their advantages and limitations are discussed.
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