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CRISPR/Cas9-mediated gene knockout of NANOG and NANOGP8 decreases the malignant
potential of prostate cancer cells
#MMPMID26087476
Kawamura N
; Nimura K
; Nagano H
; Yamaguchi S
; Nonomura N
; Kaneda Y
Oncotarget
2015[Sep]; 6
(26
): 22361-74
PMID26087476
show ga
NANOG expression in prostate cancer is highly correlated with cancer stem cell
characteristics and resistance to androgen deprivation. However, it is not clear
whether NANOG or its pseudogenes contribute to the malignant potential of cancer.
We established NANOG- and NANOGP8-knockout DU145 prostate cancer cell lines using
the CRISPR/Cas9 system. Knockouts of NANOG and NANOGP8 significantly attenuated
malignant potential, including sphere formation, anchorage-independent growth,
migration capability, and drug resistance, compared to parental DU145 cells.
NANOG and NANOGP8 knockout did not inhibit in vitro cell proliferation, but in
vivo tumorigenic potential decreased significantly. These phenotypes were
recovered in NANOG- and NANOGP8-rescued cell lines. These results indicate that
NANOG and NANOGP8 proteins are expressed in prostate cancer cell lines, and NANOG
and NANOGP8 equally contribute to the high malignant potential of prostate
cancer.
|*CRISPR-Cas Systems
[MESH]
|*Clustered Regularly Interspaced Short Palindromic Repeats
[MESH]
free
free
free
