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10.1111/mmi.12941 http://scihub22266oqcxt.onion/10.1111/mmi.12941 C4671257!4671257 !25599609     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25599609 &cmd=llinks): Failed to open stream: HTTP request failed! 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Plasmodium falciparum?SERA5 plays a non-enzymatic role in the malarial asexual blood-stage lifecycle |pdf=|usr=}}{{25599609 }} gab.com Text Plasmodium falciparum SERA5 plays a non-enzymatic role in the malarial asexual blood-stage lifecycle JO: Mol Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=25599609 #FOAvip The malaria parasite Plasmodium falciparum replicates in an intraerythrocytic parasitophorous vacuole (PV). The most abundant P.?falciparum?PV protein, called SERA5, is essential in blood stages and possesses a papain-like domain, prompting speculation that it functions as a proteolytic enzyme. Unusually however, SERA5 possesses a Ser residue (Ser596) at the position of the canonical catalytic Cys of papain-like proteases, and the function of SERA5 or whether it performs an enzymatic role is unknown. In this study, we failed to detect proteolytic activity associated with the Ser596-containing parasite-derived or recombinant protein. However, substitution of Ser596 with a Cys residue produced an active recombinant enzyme with characteristics of a cysteine protease, demonstrating that SERA5 can bind peptides. Using targeted homologous recombination in P.?falciparum, we substituted Ser596 with Ala with no phenotypic consequences, proving that SERA5 does not perform an essential enzymatic role in the parasite. We could also replace an internal segment of SERA5 with an affinity-purification tag. In contrast, using almost identical targeting constructs, we could not truncate or C-terminally tag the SERA5 gene, or replace Ser596 with a bulky Arg residue. Our findings show that SERA5 plays an indispensable but non-enzymatic role in the P.?falciparum blood-stage life cycle. Twit Text FOAVip Plasmodium falciparum SERA5 plays a non-enzymatic role in the malarial asexual blood-stage lifecycle ????Mol Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=25599609 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25599609 #FOAvip English Wikipedia <ref>{{Cite pmid|25599609 }}</ref> Plasmodium falciparum?SERA5 plays a non-enzymatic role in the malarial asexual blood-stage lifecycle #MMPMID25599609 Stallmach R ; Kavishwar M ; Withers-Martinez C ; Hackett F ; Collins CR ; Howell SA ; Yeoh S ; Knuepfer E ; Atid AJ ; Holder AA ; Blackman MJ Mol Microbiol 2015[Apr]; 96 (2 ): 368-87 PMID25599609 show ga The malaria parasite Plasmodium falciparum replicates in an intraerythrocytic parasitophorous vacuole (PV). The most abundant P.?falciparum?PV protein, called SERA5, is essential in blood stages and possesses a papain-like domain, prompting speculation that it functions as a proteolytic enzyme. Unusually however, SERA5 possesses a Ser residue (Ser596) at the position of the canonical catalytic Cys of papain-like proteases, and the function of SERA5 or whether it performs an enzymatic role is unknown. In this study, we failed to detect proteolytic activity associated with the Ser596-containing parasite-derived or recombinant protein. However, substitution of Ser596 with a Cys residue produced an active recombinant enzyme with characteristics of a cysteine protease, demonstrating that SERA5 can bind peptides. Using targeted homologous recombination in P.?falciparum, we substituted Ser596 with Ala with no phenotypic consequences, proving that SERA5 does not perform an essential enzymatic role in the parasite. We could also replace an internal segment of SERA5 with an affinity-purification tag. In contrast, using almost identical targeting constructs, we could not truncate or C-terminally tag the SERA5 gene, or replace Ser596 with a bulky Arg residue. Our findings show that SERA5 plays an indispensable but non-enzymatic role in the P.?falciparum blood-stage life cycle. |Amino Acid Motifs [MESH] |Antigens, Protozoan/chemistry/genetics/*metabolism [MESH] |Humans [MESH] |Life Cycle Stages [MESH] |Malaria, Falciparum/blood/*parasitology [MESH] |Peptide Hydrolases/chemistry/genetics/*metabolism [MESH] |Plasmodium falciparum/enzymology/genetics/*growth & development/physiology [MESH]Plasmodium falciparum?SERA5 plays a non-enzymatic role in the malarial(epmc).pdf DeepDyve Pubget Overpricing