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10.1289/ehp.1409143 http://scihub22266oqcxt.onion/10.1289/ehp.1409143 C4671229!4671229!26018136     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Environ+Health+Perspect 2015 ; 123 (12): 1287-93 Nephropedia Template TP {{tp|p=26018136|t=2015. In Vitro Effects of Bisphenol A ?-D-Glucuronide (BPA-G) on Adipogenesis in Human and Murine Preadipocytes |pdf=|usr=}}{{26018136}} gab.com Text In Vitro Effects of Bisphenol A -D-Glucuronide (BPA-G) on Adipogenesis in Human and Murine Preadipocytes JO: Environ Health Perspect http://www.kidney.de/pget.php?&tw=1&pmid=26018136 #FOAvip Background: Exposure to common environmental substances, such as bisphenol A (BPA), has been associated with a number of negative health outcomes. In vivo, BPA is rapidly converted to its predominant metabolite, BPA-glucuronide (BPA-G), which has long been believed to be biologically inactive because it lacks estrogenic activity. However, the effects of BPA-G on cellular metabolism have not been characterized. In the present study we examined the effect of BPA-G on adipogenesis. Methods: The effect of BPA-G on the differentiation of human and 3T3L1 murine preadipocytes was evaluated in vitro by quantifying lipid accumulation and the expression of adipogenic markers. Results: Treatment of 3T3L1 preadipocytes with 10 ?M BPA-G induced a significant increase in lipid accumulation, mRNA expression of the adipogenic markers sterol regulatory element binding factor 1 (SREBF1) and lipoprotein lipase (LPL), and protein levels of LPL, aP2, and adipsin. Treatment of primary human preadipocytes with BPA-G also induced adipogenesis as determined by aP2 levels. Co-treatment of cells with the estrogen receptor (ER) antagonist fulvestrant (ICI) significantly inhibited the BPA-G?induced increase in LPL and aP2 levels, whereas treatment with ICI alone had no effect. Moreover, BPA-G did not display any significant estrogenic activity. Conclusions: To our knowledge, this study is the first to report that BPA-G induces adipocyte differentiation and is not simply an inactive metabolite. The fact that BPA-G induced adipogenesis and was inhibited by an ER antagonist yet showed no estrogenic activity suggests that it has no classical ER transcriptional activation function and acts through a pathway that remains to be determined. Citation: Boucher JG, Boudreau A, Ahmed S, Atlas E. 2015. In vitro effects of bisphenol A ?-D-glucuronide (BPA-G) on adipogenesis in human and murine preadipocytes. Environ Health Perspect 123:1287?1293;?http://dx.doi.org/10.1289/ehp.1409143 Twit Text FOAVip In Vitro Effects of Bisphenol A -D-Glucuronide (BPA-G) on Adipogenesis in Human and Murine Preadipocytes ????Environ Health Perspect http://www.kidney.de/pget.php?&tw=1&pmid=26018136 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26018136 #FOAvip English Wikipedia <ref>{{Cite pmid|26018136}}</ref> In Vitro Effects of Bisphenol A ?-D-Glucuronide (BPA-G) on Adipogenesis in Human and Murine Preadipocytes #MMPMID26018136 Boucher JG; Boudreau A; Ahmed S; Atlas E Environ Health Perspect 2015[Dec]; 123 (12): 1287-93 PMID26018136show ga Background: Exposure to common environmental substances, such as bisphenol A (BPA), has been associated with a number of negative health outcomes. In vivo, BPA is rapidly converted to its predominant metabolite, BPA-glucuronide (BPA-G), which has long been believed to be biologically inactive because it lacks estrogenic activity. However, the effects of BPA-G on cellular metabolism have not been characterized. In the present study we examined the effect of BPA-G on adipogenesis. Methods: The effect of BPA-G on the differentiation of human and 3T3L1 murine preadipocytes was evaluated in vitro by quantifying lipid accumulation and the expression of adipogenic markers. Results: Treatment of 3T3L1 preadipocytes with 10 ?M BPA-G induced a significant increase in lipid accumulation, mRNA expression of the adipogenic markers sterol regulatory element binding factor 1 (SREBF1) and lipoprotein lipase (LPL), and protein levels of LPL, aP2, and adipsin. Treatment of primary human preadipocytes with BPA-G also induced adipogenesis as determined by aP2 levels. Co-treatment of cells with the estrogen receptor (ER) antagonist fulvestrant (ICI) significantly inhibited the BPA-G?induced increase in LPL and aP2 levels, whereas treatment with ICI alone had no effect. Moreover, BPA-G did not display any significant estrogenic activity. Conclusions: To our knowledge, this study is the first to report that BPA-G induces adipocyte differentiation and is not simply an inactive metabolite. The fact that BPA-G induced adipogenesis and was inhibited by an ER antagonist yet showed no estrogenic activity suggests that it has no classical ER transcriptional activation function and acts through a pathway that remains to be determined. Citation: Boucher JG, Boudreau A, Ahmed S, Atlas E. 2015. In vitro effects of bisphenol A ?-D-glucuronide (BPA-G) on adipogenesis in human and murine preadipocytes. Environ Health Perspect 123:1287?1293;?http://dx.doi.org/10.1289/ehp.1409143 äIn Vitro Effects of Bisphenol A ?-D-Glucuronide (BPA-G) on Adipogenesi(epmc).pdf DeepDyve Pubget Overpricing