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10.1111/jmi.12223 http://scihub22266oqcxt.onion/10.1111/jmi.12223 C4670706!4670706 !25611576     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25611576 &cmd=llinks): Failed to open stream: HTTP request failed! 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Array tomography: characterizing FAC-sorted populations of zebrafish immune cells by their 3D ultrastructure |pdf=|usr=}}{{25611576 }} gab.com Text Array tomography: characterizing FAC-sorted populations of zebrafish immune cells by their 3D ultrastructure JO: J Microsc http://www.kidney.de/pget.php?&tw=1&pmid=25611576 #FOAvip For 3D reconstructions of whole immune cells from zebrafish, isolated from adult animals by FAC-sorting we employed array tomography on hundreds of serial sections deposited on silicon wafers. Image stacks were either recorded manually or automatically with the newly released ZEISS Atlas 5 Array Tomography platform on a Zeiss FEGSEM. To characterize different populations of immune cells, organelle inventories were created by segmenting individual cells. In addition, arrays were used for quantification of cell populations with respect to the various cell types they contained. The detection of immunological synapses in cocultures of cell populations from thymus or WKM with cancer cells helped to identify the cytotoxic nature of these cells. Our results demonstrate the practicality and benefit of AT for high-throughput ultrastructural imaging of substantial volumes. Twit Text FOAVip Array tomography: characterizing FAC-sorted populations of zebrafish immune cells by their 3D ultrastructure ????J Microsc http://www.kidney.de/pget.php?&tw=1&pmid=25611576 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25611576 #FOAvip English Wikipedia <ref>{{Cite pmid|25611576 }}</ref> Array tomography: characterizing FAC-sorted populations of zebrafish immune cells by their 3D ultrastructure #MMPMID25611576 Wacker I ; Chockley P ; Bartels C ; Spomer W ; Hofmann A ; Gengenbach U ; Singh S ; Thaler M ; Grabher C ; Schröder RR J Microsc 2015[Aug]; 259 (2 ): 105-113 PMID25611576 show ga For 3D reconstructions of whole immune cells from zebrafish, isolated from adult animals by FAC-sorting we employed array tomography on hundreds of serial sections deposited on silicon wafers. Image stacks were either recorded manually or automatically with the newly released ZEISS Atlas 5 Array Tomography platform on a Zeiss FEGSEM. To characterize different populations of immune cells, organelle inventories were created by segmenting individual cells. In addition, arrays were used for quantification of cell populations with respect to the various cell types they contained. The detection of immunological synapses in cocultures of cell populations from thymus or WKM with cancer cells helped to identify the cytotoxic nature of these cells. Our results demonstrate the practicality and benefit of AT for high-throughput ultrastructural imaging of substantial volumes. |Adult [MESH] |Animals [MESH] |Cell Line, Tumor [MESH] |Cell Movement [MESH] |Cell Separation [MESH] |Cells, Cultured [MESH] |Flow Cytometry/methods [MESH] |Humans [MESH] |Imaging, Three-Dimensional/*methods [MESH] |Immune System/*cytology/*ultrastructure [MESH] |Immunological Synapses/ultrastructure [MESH] |Lymphocytes/*ultrastructure [MESH] |Thymus Gland/cytology/ultrastructure [MESH] |Tomography/*methods [MESH]Array tomography: characterizing FAC-sorted populations of zebrafish i(epmc).pdf DeepDyve Pubget Overpricing