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2011 ; 11
(1-2
): 118-21
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Transgenic labeling of the zebrafish pronephric duct and tubules using a promoter
from the enpep gene
#MMPMID20969977
Seiler C
; Pack M
Gene Expr Patterns
2011[Jan]; 11
(1-2
): 118-21
PMID20969977
show ga
In recent years the zebrafish has become a popular model system to study organ
development and disease. To facilitate these studies, genetic tools are required
which allow to modify and manipulate gene expression in organs of interest. Here
we describe a zebrafish 2kb glutamyl aminopeptidase (enpep) promoter fragment,
and show that it can drive gene expression specifically in the kidney during
early and late development. We established a stable transgenic line using this
promoter fragment that has specific GFP expression in pronephric ducts and
tubules starting at 20h post-fertilization.