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10.1038/cddis.2014.556 http://scihub22266oqcxt.onion/10.1038/cddis.2014.556 C4669754!4669754 !25611389     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25611389 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Cell+Death+Dis 2015 ; 6 (1 ): e1614 Nephropedia Template TP {{tp|p=25611389 |t=2015. Genome-wide mRNA and miRNA expression profiling reveal multiple regulatory networks in colorectal cancer |pdf=|usr=}}{{25611389 }} gab.com Text Genome-wide mRNA and miRNA expression profiling reveal multiple regulatory networks in colorectal cancer JO: Cell Death Dis http://www.kidney.de/pget.php?&tw=1&pmid=25611389 #FOAvip Despite recent advances in cancer management, colorectal cancer (CRC) remains the third most common cancer and a major health-care problem worldwide. MicroRNAs have recently emerged as key regulators of cancer development and progression by targeting multiple cancer-related genes; however, such regulatory networks are not well characterized in CRC. Thus, the aim of this study was to perform global messenger RNA (mRNA) and microRNA expression profiling in the same CRC samples and adjacent normal tissues and to identify potential miRNA-mRNA regulatory networks. Our data revealed 1273 significantly upregulated and 1902 downregulated genes in CRC. Pathway analysis revealed significant enrichment in cell cycle, integrated cancer, Wnt (wingless-type MMTV integration site family member), matrix metalloproteinase, and TGF-? pathways in CRC. Pharmacological inhibition of Wnt (using XAV939 or IWP-2) or TGF-? (using SB-431542) pathways led to dose- and time-dependent inhibition of CRC cell growth. Similarly, our data revealed up- (42) and downregulated (61) microRNAs in the same matched samples. Using target prediction and bioinformatics, ~77% of the upregulated genes were predicted to be targeted by microRNAs found to be downregulated in CRC. We subsequently focused on EZH2 (enhancer of zeste homolog 2 ), which was found to be regulated by hsa-miR-26a-5p and several members of the let-7 (lethal-7) family in CRC. Significant inverse correlation between EZH2 and hsa-miR-26a-5p (R(2)=0.56, P=0.0001) and hsa-let-7b-5p (R(2)=0.19, P=0.02) expression was observed in the same samples, corroborating the belief of EZH2 being a bona fide target for these two miRNAs in CRC. Pharmacological inhibition of EZH2 led to significant reduction in trimethylated histone H3 on lysine 27 (H3K27) methylation, marked reduction in cell proliferation, and migration in vitro. Concordantly, small interfering RNA-mediated knockdown of EZH2 led to similar effects on CRC cell growth in vitro. Therefore, our data have revealed several hundred potential miRNA-mRNA regulatory networks in CRC and suggest targeting relevant networks as potential therapeutic strategy for CRC. Twit Text FOAVip Genome-wide mRNA and miRNA expression profiling reveal multiple regulatory networks in colorectal cancer ????Cell Death Dis http://www.kidney.de/pget.php?&tw=1&pmid=25611389 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25611389 #FOAvip English Wikipedia <ref>{{Cite pmid|25611389 }}</ref> Genome-wide mRNA and miRNA expression profiling reveal multiple regulatory networks in colorectal cancer #MMPMID25611389 Vishnubalaji R ; Hamam R ; Abdulla MH ; Mohammed MA ; Kassem M ; Al-Obeed O ; Aldahmash A ; Alajez NM Cell Death Dis 2015[Jan]; 6 (1 ): e1614 PMID25611389 show ga Despite recent advances in cancer management, colorectal cancer (CRC) remains the third most common cancer and a major health-care problem worldwide. MicroRNAs have recently emerged as key regulators of cancer development and progression by targeting multiple cancer-related genes; however, such regulatory networks are not well characterized in CRC. Thus, the aim of this study was to perform global messenger RNA (mRNA) and microRNA expression profiling in the same CRC samples and adjacent normal tissues and to identify potential miRNA-mRNA regulatory networks. Our data revealed 1273 significantly upregulated and 1902 downregulated genes in CRC. Pathway analysis revealed significant enrichment in cell cycle, integrated cancer, Wnt (wingless-type MMTV integration site family member), matrix metalloproteinase, and TGF-? pathways in CRC. Pharmacological inhibition of Wnt (using XAV939 or IWP-2) or TGF-? (using SB-431542) pathways led to dose- and time-dependent inhibition of CRC cell growth. Similarly, our data revealed up- (42) and downregulated (61) microRNAs in the same matched samples. Using target prediction and bioinformatics, ~77% of the upregulated genes were predicted to be targeted by microRNAs found to be downregulated in CRC. We subsequently focused on EZH2 (enhancer of zeste homolog 2 ), which was found to be regulated by hsa-miR-26a-5p and several members of the let-7 (lethal-7) family in CRC. Significant inverse correlation between EZH2 and hsa-miR-26a-5p (R(2)=0.56, P=0.0001) and hsa-let-7b-5p (R(2)=0.19, P=0.02) expression was observed in the same samples, corroborating the belief of EZH2 being a bona fide target for these two miRNAs in CRC. Pharmacological inhibition of EZH2 led to significant reduction in trimethylated histone H3 on lysine 27 (H3K27) methylation, marked reduction in cell proliferation, and migration in vitro. Concordantly, small interfering RNA-mediated knockdown of EZH2 led to similar effects on CRC cell growth in vitro. Therefore, our data have revealed several hundred potential miRNA-mRNA regulatory networks in CRC and suggest targeting relevant networks as potential therapeutic strategy for CRC. |*Gene Expression Profiling [MESH] |*Gene Expression Regulation, Neoplastic/drug effects [MESH] |*Gene Regulatory Networks/drug effects [MESH] |Adenosine/analogs & derivatives/pharmacology [MESH] |Adult [MESH] |Aged [MESH] |Base Sequence [MESH] |Cell Movement/drug effects/genetics [MESH] |Cell Proliferation/drug effects [MESH] |Cell Survival/drug effects [MESH] |Colorectal Neoplasms/*genetics/pathology [MESH] |Enhancer of Zeste Homolog 2 Protein [MESH] |Female [MESH] |Genome, Human [MESH] |Humans [MESH] |Male [MESH] |MicroRNAs/*genetics/metabolism [MESH] |Middle Aged [MESH] |Molecular Sequence Data [MESH] |Polycomb Repressive Complex 2/metabolism [MESH]Genome-wide mRNA and miRNA expression profiling reveal multiple regula(epmc).pdf DeepDyve Pubget Overpricing