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http://scihub22266oqcxt.onion/ C4668827!4668827!25967027     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Appl+Opt 2015 ; 54 (1): A89-97 Nephropedia Template TP {{tp|p=25967027|t=2015. Digital Holography of Intracellular Dynamics to Probe Tissue Physiology |pdf=|usr=}}{{25967027}} gab.com Text Digital Holography of Intracellular Dynamics to Probe Tissue Physiology JO: Appl Opt http://www.kidney.de/pget.php?&tw=1&pmid=25967027 #FOAvip Digital holography provides improved capabilities for imaging through dense tissue. Using a short-coherence source, the digital hologram recorded from backscattered light performs laser ranging that maintains fidelity of information acquired from depths much greater than possible by traditional imaging techniques. Biodynamic Imaging (BDI) is a developing technology for live-tissue imaging of up to a millimeter in depth that uses the hologram intensity fluctuations as label-free image contrast and can study tissue behavior in native microenvironments. In this paper BDI is used investigate the change in adhesion-dependent tissue response in 3D cultures. The results show that increasing density of cellular adhesions slows motion inside tissue and alters the response to cytoskeletal drugs. A clear signature of membrane fluctuations was observed in mid frequencies (0.1 ? 1 Hz) that was enhanced by the application of cytochalasin-D that degrades the actin cortex inside the cell membrane. This enhancement feature is only observed in tissues that have formed adhesions, because cell pellets initially do not show this signature, but develop this signature only after incubation enables adhesions to form. Twit Text FOAVip Digital Holography of Intracellular Dynamics to Probe Tissue Physiology ????Appl Opt http://www.kidney.de/pget.php?&tw=1&pmid=25967027 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25967027 #FOAvip English Wikipedia <ref>{{Cite pmid|25967027}}</ref> Digital Holography of Intracellular Dynamics to Probe Tissue Physiology #MMPMID25967027 Merrill D; An R; Turek J; Nolte DD Appl Opt 2015[Jan]; 54 (1): A89-97 PMID25967027show ga Digital holography provides improved capabilities for imaging through dense tissue. Using a short-coherence source, the digital hologram recorded from backscattered light performs laser ranging that maintains fidelity of information acquired from depths much greater than possible by traditional imaging techniques. Biodynamic Imaging (BDI) is a developing technology for live-tissue imaging of up to a millimeter in depth that uses the hologram intensity fluctuations as label-free image contrast and can study tissue behavior in native microenvironments. In this paper BDI is used investigate the change in adhesion-dependent tissue response in 3D cultures. The results show that increasing density of cellular adhesions slows motion inside tissue and alters the response to cytoskeletal drugs. A clear signature of membrane fluctuations was observed in mid frequencies (0.1 ? 1 Hz) that was enhanced by the application of cytochalasin-D that degrades the actin cortex inside the cell membrane. This enhancement feature is only observed in tissues that have formed adhesions, because cell pellets initially do not show this signature, but develop this signature only after incubation enables adhesions to form. äDigital Holography of Intracellular Dynamics to Probe Tissue Physiolog(epmc).pdf DeepDyve Pubget Overpricing