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10.1038/mtm.2015.47 http://scihub22266oqcxt.onion/10.1038/mtm.2015.47 C4667716!4667716!26665132     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Ther+Methods+Clin+Dev 2015 ; 2 (ä): 15047- Nephropedia Template TP {{tp|p=26665132|t=2015. Super-resolution imaging of nuclear import of adeno-associated virus in live cells |pdf=|usr=}}{{26665132}} gab.com Text Super-resolution imaging of nuclear import of adeno-associated virus in live cells JO: Mol Ther Methods Clin Dev http://www.kidney.de/pget.php?&tw=1&pmid=26665132 #FOAvip Adeno-associated virus (AAV) has been developed as a promising human gene therapy vector. Particularly, recombinant AAV vector (rAAV) achieves its transduction of host cells by crossing at least three physiological barriers including plasma membrane, endosomal membrane, and nuclear envelope (NE). So far, the AAV transduction mechanism has not been explored thoroughly at the single viral particle level. In this study, we employed high-speed super-resolution single-point edge-excitation sub-diffraction (SPEED) microscopy to map the events of single rAAV2 particles infecting live human cells with an unprecedented spatiotemporal resolution of 9?12?nm and 2?20?ms. Data reveal that rAAV2 particles are imported through nuclear pore complexes (NPCs) rather than nuclear membrane budding into the nucleus. Moreover, approximately 17% of the rAAV2 molecules starting from the cytoplasm successfully transverse the NPCs to reach the nucleoplasm, revealing that the NPCs act as a strict selective step for AAV delivery. This study lastly suggests a new pathway to improve AAV vectors for human gene therapy. Twit Text FOAVip Super-resolution imaging of nuclear import of adeno-associated virus in live cells ????Mol Ther Methods Clin Dev http://www.kidney.de/pget.php?&tw=1&pmid=26665132 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26665132 #FOAvip English Wikipedia <ref>{{Cite pmid|26665132}}</ref> Super-resolution imaging of nuclear import of adeno-associated virus in live cells #MMPMID26665132 Kelich JM; Ma J; Dong B; Wang Q; Chin M; Magura CM; Xiao W; Yang W Mol Ther Methods Clin Dev 2015[]; 2 (ä): 15047- PMID26665132show ga Adeno-associated virus (AAV) has been developed as a promising human gene therapy vector. Particularly, recombinant AAV vector (rAAV) achieves its transduction of host cells by crossing at least three physiological barriers including plasma membrane, endosomal membrane, and nuclear envelope (NE). So far, the AAV transduction mechanism has not been explored thoroughly at the single viral particle level. In this study, we employed high-speed super-resolution single-point edge-excitation sub-diffraction (SPEED) microscopy to map the events of single rAAV2 particles infecting live human cells with an unprecedented spatiotemporal resolution of 9?12?nm and 2?20?ms. Data reveal that rAAV2 particles are imported through nuclear pore complexes (NPCs) rather than nuclear membrane budding into the nucleus. Moreover, approximately 17% of the rAAV2 molecules starting from the cytoplasm successfully transverse the NPCs to reach the nucleoplasm, revealing that the NPCs act as a strict selective step for AAV delivery. This study lastly suggests a new pathway to improve AAV vectors for human gene therapy. äSuper-resolution imaging of nuclear import of adeno-associated virus i(epmc).pdf DeepDyve Pubget Overpricing