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10.1038/nmeth.3619 http://scihub22266oqcxt.onion/10.1038/nmeth.3619 C4666781!4666781!26480475     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Methods 2015 ; 12 (12): 1197-204 Nephropedia Template TP {{tp|p=26480475|t=2015. Tissue matrix arrays for high throughput screening and systems analysis of cell function |pdf=|usr=}}{{26480475}} gab.com Text Tissue matrix arrays for high throughput screening and systems analysis of cell function JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=26480475 #FOAvip Cell and protein arrays have demonstrated remarkable utility in the high-throughput evaluation of biological responses; however, they lack the complexity of native tissue and organs. Here, we describe tissue extracellular matrix (ECM) arrays for screening biological outputs and systems analysis. We spotted processed tissue ECM particles as two-dimensional arrays or incorporated them with cells to generate three-dimensional cell-matrix microtissue arrays. We then investigated the response of human stem, cancer, and immune cells to tissue ECM arrays originating from 11 different tissues, and validated the 2D and 3D arrays as representative of the in vivo microenvironment through quantitative analysis of tissue-specific cellular responses, including matrix production, adhesion and proliferation, and morphological changes following culture. The biological outputs correlated with tissue proteomics, and network analysis identified several proteins linked to cell function. Our methodology enables broad screening of ECMs to connect tissue-specific composition with biological activity, providing a new resource for biomaterials research and translation. Twit Text FOAVip Tissue matrix arrays for high throughput screening and systems analysis of cell function ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=26480475 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26480475 #FOAvip English Wikipedia <ref>{{Cite pmid|26480475}}</ref> Tissue matrix arrays for high throughput screening and systems analysis of cell function #MMPMID26480475 Beachley VZ; Wolf MT; Sadtler K; Manda SS; Jacobs H; Blatchley M; Bader JS; Pandey A; Pardoll D; Elisseeff JH Nat Methods 2015[Dec]; 12 (12): 1197-204 PMID26480475show ga Cell and protein arrays have demonstrated remarkable utility in the high-throughput evaluation of biological responses; however, they lack the complexity of native tissue and organs. Here, we describe tissue extracellular matrix (ECM) arrays for screening biological outputs and systems analysis. We spotted processed tissue ECM particles as two-dimensional arrays or incorporated them with cells to generate three-dimensional cell-matrix microtissue arrays. We then investigated the response of human stem, cancer, and immune cells to tissue ECM arrays originating from 11 different tissues, and validated the 2D and 3D arrays as representative of the in vivo microenvironment through quantitative analysis of tissue-specific cellular responses, including matrix production, adhesion and proliferation, and morphological changes following culture. The biological outputs correlated with tissue proteomics, and network analysis identified several proteins linked to cell function. Our methodology enables broad screening of ECMs to connect tissue-specific composition with biological activity, providing a new resource for biomaterials research and translation. äTissue matrix arrays for high throughput screening and systems analysi(epmc).pdf DeepDyve Pubget Overpricing