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10.1038/nmeth.3626 http://scihub22266oqcxt.onion/10.1038/nmeth.3626 C4666732!4666732!26480474     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Methods 2015 ; 12 (12): 1191-6 Nephropedia Template TP {{tp|p=26480474|t=2015. Continuously Tunable Nucleic Acid Hybridization Probes |pdf=|usr=}}{{26480474}} gab.com Text Continuously Tunable Nucleic Acid Hybridization Probes JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=26480474 #FOAvip In silico designed nucleic acid probes and primers often fail to achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. Here, we present a novel, on-the-fly method of tuning probe affinity and selectivity via the stoichiometry of auxiliary species, allowing independent and decoupled adjustment of hybridization yield for different probes in multiplexed assays. Using this method, we achieve near-continuous tuning of probe effective free energy (0.03 kcal·mol?1 granularity). As applications, we enforced uniform capture efficiency of 31 DNA molecules (GC content 0% ? 100%), maximized signal difference for 11 pairs of single nucleotide variants, and performed tunable hybrid-capture of mRNA from total RNA. Using the Nanostring nCounter platform, we applied stoichiometric tuning to simultaneously adjust yields for a 24-plex assay, and we show multiplexed quantitation of RNA sequences and variants from formalin-fixed, paraffin-embedded samples (FFPE). Twit Text FOAVip Continuously Tunable Nucleic Acid Hybridization Probes ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=26480474 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26480474 #FOAvip English Wikipedia <ref>{{Cite pmid|26480474}}</ref> Continuously Tunable Nucleic Acid Hybridization Probes #MMPMID26480474 Wu LR; Wang JS; Fang JZ; Reiser E; Pinto A; Pekker I; Boykin R; Ngouenet C; Webster PJ; Beechem J; Zhang DY Nat Methods 2015[Dec]; 12 (12): 1191-6 PMID26480474show ga In silico designed nucleic acid probes and primers often fail to achieve favorable specificity and sensitivity tradeoffs on the first try, and iterative empirical sequence-based optimization is needed, particularly in multiplexed assays. Here, we present a novel, on-the-fly method of tuning probe affinity and selectivity via the stoichiometry of auxiliary species, allowing independent and decoupled adjustment of hybridization yield for different probes in multiplexed assays. Using this method, we achieve near-continuous tuning of probe effective free energy (0.03 kcal·mol?1 granularity). As applications, we enforced uniform capture efficiency of 31 DNA molecules (GC content 0% ? 100%), maximized signal difference for 11 pairs of single nucleotide variants, and performed tunable hybrid-capture of mRNA from total RNA. Using the Nanostring nCounter platform, we applied stoichiometric tuning to simultaneously adjust yields for a 24-plex assay, and we show multiplexed quantitation of RNA sequences and variants from formalin-fixed, paraffin-embedded samples (FFPE). äContinuously Tunable Nucleic Acid Hybridization Probes (epmc).pdf DeepDyve Pubget Overpricing