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10.1038/nmeth.3580 http://scihub22266oqcxt.onion/10.1038/nmeth.3580 C4666719!4666719!26344044     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Methods 2015 ; 12 (11): 1051-4 Nephropedia Template TP {{tp|p=26344044|t=2015. Cas9 gRNA engineering for genome editing, activation and repression |pdf=|usr=}}{{26344044}} gab.com Text Cas9 gRNA engineering for genome editing, activation and repression JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=26344044 #FOAvip We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein. Twit Text FOAVip Cas9 gRNA engineering for genome editing, activation and repression ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=26344044 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26344044 #FOAvip English Wikipedia <ref>{{Cite pmid|26344044}}</ref> Cas9 gRNA engineering for genome editing, activation and repression #MMPMID26344044 Kiani S; Chavez A; Tuttle M; Hall RN; Chari R; Ter-Ovanesyan D; Qian J; Pruitt BW; Beal J; Vora S; Buchthal J; Kowal EJK; Ebrahimkhani MR; Collins JJ; Weiss R; Church G Nat Methods 2015[Nov]; 12 (11): 1051-4 PMID26344044show ga We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein. äCas9 gRNA engineering for genome editing, activation and repression (epmc).pdf DeepDyve Pubget Overpricing