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10.1371/journal.ppat.1005281 http://scihub22266oqcxt.onion/10.1371/journal.ppat.1005281 C4666624!4666624!26625259     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+Pathog 2015 ; 11 (12): ä Nephropedia Template TP {{tp|p=26625259|t=2015. The SUMOylation Pathway Restricts Gene Transduction by Adeno-Associated Viruses |pdf=|usr=}}{{26625259}} gab.com Text The SUMOylation Pathway Restricts Gene Transduction by Adeno-Associated Viruses JO: PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=26625259 #FOAvip Adeno-associated viruses are members of the genus dependoviruses of the parvoviridae family. AAV vectors are considered promising vectors for gene therapy and genetic vaccination as they can be easily produced, are highly stable and non-pathogenic. Nevertheless, transduction of cells in vitro and in vivo by AAV in the absence of a helper virus is comparatively inefficient requiring high multiplicity of infection. Several bottlenecks for AAV transduction have previously been described, including release from endosomes, nuclear transport and conversion of the single stranded DNA into a double stranded molecule. We hypothesized that the bottlenecks in AAV transduction are, in part, due to the presence of host cell restriction factors acting directly or indirectly on the AAV-mediated gene transduction. In order to identify such factors we performed a whole genome siRNA screen which identified a number of putative genes interfering with AAV gene transduction. A number of factors, yielding the highest scores, were identified as members of the SUMOylation pathway. We identified Ubc9, the E2 conjugating enzyme as well as Sae1 and Sae2, enzymes responsible for activating E1, as factors involved in restricting AAV. The restriction effect, mediated by these factors, was validated and reproduced independently. Our data indicate that SUMOylation targets entry of AAV capsids and not downstream processes of uncoating, including DNA single strand conversion or DNA damage signaling. We suggest that transiently targeting SUMOylation will enhance application of AAV in vitro and in vivo. Twit Text FOAVip The SUMOylation Pathway Restricts Gene Transduction by Adeno-Associated Viruses ????PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=26625259 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26625259 #FOAvip English Wikipedia <ref>{{Cite pmid|26625259}}</ref> The SUMOylation Pathway Restricts Gene Transduction by Adeno-Associated Viruses #MMPMID26625259 Hölscher C; Sonntag F; Henrich K; Chen Q; Beneke J; Matula P; Rohr K; Kaderali L; Beil N; Erfle H; Kleinschmidt JA; Müller M PLoS Pathog 2015[Dec]; 11 (12): ä PMID26625259show ga Adeno-associated viruses are members of the genus dependoviruses of the parvoviridae family. AAV vectors are considered promising vectors for gene therapy and genetic vaccination as they can be easily produced, are highly stable and non-pathogenic. Nevertheless, transduction of cells in vitro and in vivo by AAV in the absence of a helper virus is comparatively inefficient requiring high multiplicity of infection. Several bottlenecks for AAV transduction have previously been described, including release from endosomes, nuclear transport and conversion of the single stranded DNA into a double stranded molecule. We hypothesized that the bottlenecks in AAV transduction are, in part, due to the presence of host cell restriction factors acting directly or indirectly on the AAV-mediated gene transduction. In order to identify such factors we performed a whole genome siRNA screen which identified a number of putative genes interfering with AAV gene transduction. A number of factors, yielding the highest scores, were identified as members of the SUMOylation pathway. We identified Ubc9, the E2 conjugating enzyme as well as Sae1 and Sae2, enzymes responsible for activating E1, as factors involved in restricting AAV. The restriction effect, mediated by these factors, was validated and reproduced independently. Our data indicate that SUMOylation targets entry of AAV capsids and not downstream processes of uncoating, including DNA single strand conversion or DNA damage signaling. We suggest that transiently targeting SUMOylation will enhance application of AAV in vitro and in vivo. äThe SUMOylation Pathway Restricts Gene Transduction by Adeno-Associate(epmc).pdf DeepDyve Pubget Overpricing