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2015 ; 9
(12
): e0004251
Nephropedia Template TP
gab.com Text
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English Wikipedia
Leptospira Serovars for Diagnosis of Leptospirosis in Humans and Animals in
Africa: Common Leptospira Isolates and Reservoir Hosts
#MMPMID26624890
Mgode GF
; Machang'u RS
; Mhamphi GG
; Katakweba A
; Mulungu LS
; Durnez L
; Leirs H
; Hartskeerl RA
; Belmain SR
PLoS Negl Trop Dis
2015[Dec]; 9
(12
): e0004251
PMID26624890
show ga
The burden of leptospirosis in humans and animals in Africa is higher than that
reported from other parts of the world. However, the disease is not routinely
diagnosed in the continent. One of major factors limiting diagnosis is the poor
availability of live isolates of locally circulating Leptospira serovars for
inclusion in the antigen panel of the gold standard microscopic agglutination
test (MAT) for detecting antibodies against leptospirosis. To gain insight in
Leptospira serovars and their natural hosts occurring in Tanzania, concomitantly
enabling the improvement of the MAT by inclusion of fresh local isolates, a total
of 52 Leptospira isolates were obtained from fresh urine and kidney homogenates,
collected between 1996 and 2006 from small mammals, cattle and pigs. Isolates
were identified by serogrouping, cross agglutination absorption test (CAAT), and
molecular typing. Common Leptospira serovars with their respective animal hosts
were: Sokoine (cattle and rodents); Kenya (rodents and shrews); Mwogolo
(rodents); Lora (rodents); Qunjian (rodent); serogroup Grippotyphosa (cattle);
and an unknown serogroup from pigs. Inclusion of local serovars particularly
serovar Sokoine in MAT revealed a 10-fold increase in leptospirosis prevalence in
Tanzania from 1.9% to 16.9% in rodents and 0.26% to 10.75% in humans. This
indicates that local serovars are useful for diagnosis of human and animal
leptospirosis in Tanzania and other African countries.