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2016 ; 2016
(ä): 9062638
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Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose
Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats
#MMPMID26649053
Zhao X
; Qiu X
; Zhang Y
; Zhang S
; Gu X
; Guo H
Stem Cells Int
2016[]; 2016
(ä): 9062638
PMID26649053
show ga
It has been shown that administration of adipose derived mesenchymal stem cells
(AdMSCs) enhanced structural and functional recovery of renal
ischemia-reperfusion (IR) injury. Low engraftment of stem cells, however, limits
the therapeutic effects of AdMSCs. The present study was designed to enhance the
therapeutic effects of AdMSCs by delivering AdMSCs in a three-dimensional (3D)
aggregates form. Microwell was used to produce 3D AdMSCs aggregates. In vitro
data indicated that AdMSCs in 3D aggregates were less susceptible to oxidative
and hypoxia stress induced by 200??M peroxide and hypoxia/reoxygenation,
respectively, compared with those cultured in two-dimensional (2D) monolayer.
Furthermore, AdMSCs in 3D aggregates secreted more proangiogenic factors than
those cultured in 2D monolayer. 2D AdMSCs or 3D AdMSCs aggregates were injected
into renal cortex immediately after induction of renal IR injury. In vivo data
revealed that 3D aggregates enhanced the effects of AdMSCs in recovering function
and structure after renal IR injury. Improved grafted AdMSCs were observed in
kidney injected with 3D aggregates compared with AdMSCs cultured in 2D monolayer.
Our results demonstrated that 3D AdMSCs aggregated produced by microwell enhanced
the retention and therapeutic effects of AdMSCs for renal IR injury.