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10.1038/nature15760 http://scihub22266oqcxt.onion/10.1038/nature15760 C4662619!4662619!26503043     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nature 2015 ; 527 (7579): 535-8 Nephropedia Template TP {{tp|p=26503043|t=2015. Foreign DNA capture during CRISPR?Cas adaptive immunity |pdf=|usr=}}{{26503043}} gab.com Text Foreign DNA capture during CRISPR Cas adaptive immunity JO: Nature http://www.kidney.de/pget.php?&tw=1&pmid=26503043 #FOAvip Bacteria and archaea generate adaptive immunity against phages and plasmids by integrating foreign DNA of specific 30?40 base pair (bp) lengths into clustered regularly interspaced short palindromic repeats (CRISPR) loci as spacer segments1?6. The universally conserved Cas1?Cas2 integrase complex catalyzes spacer acquisition using a direct nucleophilic integration mechanism similar to retroviral integrases and transposases7?13. How the Cas1?Cas2 complex selects foreign DNA substrates for integration remains unknown. Here we present X-ray crystal structures of the Escherichia coli Cas1?Cas2 complex bound to cognate 33 nucleotide (nt) protospacer DNA substrates. The protein complex creates a curved binding surface spanning the length of the DNA and splays the ends of the protospacer to allow each terminal nucleophilic 3??OH to enter a channel leading into the Cas1 active sites. Phosphodiester backbone interactions between the protospacer and the proteins explain the sequence-nonspecific substrate selection observed in vivo2?4. Our results uncover the structural basis for foreign DNA capture and the mechanism by which Cas1?Cas2 functions as a molecular ruler to dictate the sequence architecture of CRISPR loci. Twit Text FOAVip Foreign DNA capture during CRISPR Cas adaptive immunity ????Nature http://www.kidney.de/pget.php?&tw=1&pmid=26503043 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26503043 #FOAvip English Wikipedia <ref>{{Cite pmid|26503043}}</ref> Foreign DNA capture during CRISPR?Cas adaptive immunity #MMPMID26503043 Nuņez JK; Harrington LB; Kranzusch PJ; Engelman AN; Doudna JA Nature 2015[Nov]; 527 (7579): 535-8 PMID26503043show ga Bacteria and archaea generate adaptive immunity against phages and plasmids by integrating foreign DNA of specific 30?40 base pair (bp) lengths into clustered regularly interspaced short palindromic repeats (CRISPR) loci as spacer segments1?6. The universally conserved Cas1?Cas2 integrase complex catalyzes spacer acquisition using a direct nucleophilic integration mechanism similar to retroviral integrases and transposases7?13. How the Cas1?Cas2 complex selects foreign DNA substrates for integration remains unknown. Here we present X-ray crystal structures of the Escherichia coli Cas1?Cas2 complex bound to cognate 33 nucleotide (nt) protospacer DNA substrates. The protein complex creates a curved binding surface spanning the length of the DNA and splays the ends of the protospacer to allow each terminal nucleophilic 3??OH to enter a channel leading into the Cas1 active sites. Phosphodiester backbone interactions between the protospacer and the proteins explain the sequence-nonspecific substrate selection observed in vivo2?4. Our results uncover the structural basis for foreign DNA capture and the mechanism by which Cas1?Cas2 functions as a molecular ruler to dictate the sequence architecture of CRISPR loci. äForeign DNA capture during CRISPR?Cas adaptive immunity (epmc).pdf DeepDyve Pubget Overpricing