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10.1016/j.devcel.2015.10.015 http://scihub22266oqcxt.onion/10.1016/j.devcel.2015.10.015 C4662609!4662609!26585297     free     free     free       Dev+Cell 2015 ; 35 (4): 497-512 Nephropedia Template TP {{tp|p=26585297|t=2015. Proteomics of Primary Cilia by Proximity Labeling |pdf=|usr=}}{{26585297}} gab.com Text Proteomics of Primary Cilia by Proximity Labeling JO: Dev Cell http://www.kidney.de/pget.php?&tw=1&pmid=26585297 #FOAvip While cilia are recognized as important signaling organelles, the extent of ciliary functions remains unknown because of difficulties in cataloguing proteins from mammalian primary cilia. We present a method that readily captures rapid snapshots of the ciliary proteome by selectively biotinylating ciliary proteins using a cilia-targeted proximity labeling enzyme (cilia-APEX). Besides identifying known ciliary proteins, cilia-APEX uncovered several ciliary signaling molecules. The kinases PKA, AMPK and LKB1 were validated as bona fide ciliary proteins and PKA was found to regulate Hedgehog signaling in primary cilia. Furthermore, proteomics profiling of Ift27/Bbs19 mutant cilia correctly detected BBSome accumulation inside Ift27?/? cilia and revealed that ?-arrestin 2 and the viral receptor CAR are candidate cargoes of the BBSome. This work demonstrates that proximity labeling can be applied to proteomics of non-membrane-enclosed organelles and suggests that proteomics profiling of cilia will enable a rapid and powerful characterization of ciliopathies. Twit Text FOAVip Proteomics of Primary Cilia by Proximity Labeling ????Dev Cell http://www.kidney.de/pget.php?&tw=1&pmid=26585297 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26585297 #FOAvip English Wikipedia <ref>{{Cite pmid|26585297}}</ref> Proteomics of Primary Cilia by Proximity Labeling #MMPMID26585297 Mick DU; Rodrigues RB; Leib RD; Adams CM; Chien AS; Gygi SP; Nachury MV Dev Cell 2015[Nov]; 35 (4): 497-512 PMID26585297show ga While cilia are recognized as important signaling organelles, the extent of ciliary functions remains unknown because of difficulties in cataloguing proteins from mammalian primary cilia. We present a method that readily captures rapid snapshots of the ciliary proteome by selectively biotinylating ciliary proteins using a cilia-targeted proximity labeling enzyme (cilia-APEX). Besides identifying known ciliary proteins, cilia-APEX uncovered several ciliary signaling molecules. The kinases PKA, AMPK and LKB1 were validated as bona fide ciliary proteins and PKA was found to regulate Hedgehog signaling in primary cilia. Furthermore, proteomics profiling of Ift27/Bbs19 mutant cilia correctly detected BBSome accumulation inside Ift27?/? cilia and revealed that ?-arrestin 2 and the viral receptor CAR are candidate cargoes of the BBSome. This work demonstrates that proximity labeling can be applied to proteomics of non-membrane-enclosed organelles and suggests that proteomics profiling of cilia will enable a rapid and powerful characterization of ciliopathies. äProteomics of Primary Cilia by Proximity Labeling (epmc).pdf DeepDyve Pubget Overpricing