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2015 ; 5
(ä): 17179
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Analysis of volatile organic compounds in exhaled breath to diagnose
ventilator-associated pneumonia
#MMPMID26608483
Schnabel R
; Fijten R
; Smolinska A
; Dallinga J
; Boumans ML
; Stobberingh E
; Boots A
; Roekaerts P
; Bergmans D
; van Schooten FJ
Sci Rep
2015[Nov]; 5
(ä): 17179
PMID26608483
show ga
Ventilator-associated pneumonia (VAP) is a nosocomial infection occurring in the
intensive care unit (ICU). The diagnostic standard is based on clinical criteria
and bronchoalveolar lavage (BAL). Exhaled breath analysis is a promising
non-invasive method for rapid diagnosis of diseases and contains volatile organic
compounds (VOCs) that can differentiate diseased from healthy individuals. The
aim of this study was to determine whether analysis of VOCs in exhaled breath can
be used as a non-invasive monitoring tool for VAP. One hundred critically ill
patients with clinical suspicion of VAP underwent BAL. Before BAL, exhaled air
samples were collected and analysed by gas chromatography time-of-flight mass
spectrometry (GC-tof-MS). The clinical suspicion of VAP was confirmed by BAL
diagnostic criteria in 32 patients [VAP(+)] and rejected in 68 patients [VAP(-)].
Multivariate statistical comparison of VOC profiles between VAP(+) and VAP(-)
revealed a subset of 12 VOCs that correctly discriminated between those two
patient groups with a sensitivity and specificity of 75.8% ± 13.5% and 73.0% ±
11.8%, respectively. These results suggest that detection of VAP in ICU patients
is possible by examining exhaled breath, enabling a simple, safe and non-invasive
approach that could diminish diagnostic burden of VAP.