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10.1126/science.aab3500 http://scihub22266oqcxt.onion/10.1126/science.aab3500 C4659358!4659358!26315442     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26315442.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Science 2015 ; 349 (6251): aab3500 Nephropedia Template TP {{tp|p=26315442|t=2015. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics |pdf=|usr=}}{{26315442}} gab.com Text Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics JO: Science http://www.kidney.de/pget.php?&tw=1&pmid=26315442 #FOAvip Super-resolution fluorescence microscopy is distinct among nanoscale imaging tools in its ability to image protein dynamics in living cells. Structured illumination microscopy (SIM) stands out in this regard because of its high speed and low illumination intensities, but typically offers only a twofold resolution gain. We extended the resolution of live-cell SIM through two approaches: ultrahigh numerical aperture SIM at 84-nanometer lateral resolution for more than 100 multicolor frames, and nonlinear SIM with patterned activation at 45- to 62-nanometer resolution for approximately 20 to 40 frames. We applied these approaches to image dynamics near the plasma membrane of spatially resolved assemblies of clathrin and caveolin, Rab5a in early endosomes, and a-actinin, often in relationship to cortical actin. In addition, we examined mitochondria, actin, and the Golgi apparatus dynamics in three dimensions. Twit Text FOAVip Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics ????Science http://www.kidney.de/pget.php?&tw=1&pmid=26315442 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26315442 #FOAvip English Wikipedia <ref>{{Cite pmid|26315442}}</ref> Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics #MMPMID26315442 Li D; Shao L; Chen BC; Zhang X; Zhang M; Moses B; Milkie DE; Beach JR; Hammer JA; Pasham M; Kirchhausen T; Baird MA; Davidson MW; Xu P; Betzig E Science 2015[Aug]; 349 (6251): aab3500 PMID26315442show ga Super-resolution fluorescence microscopy is distinct among nanoscale imaging tools in its ability to image protein dynamics in living cells. Structured illumination microscopy (SIM) stands out in this regard because of its high speed and low illumination intensities, but typically offers only a twofold resolution gain. We extended the resolution of live-cell SIM through two approaches: ultrahigh numerical aperture SIM at 84-nanometer lateral resolution for more than 100 multicolor frames, and nonlinear SIM with patterned activation at 45- to 62-nanometer resolution for approximately 20 to 40 frames. We applied these approaches to image dynamics near the plasma membrane of spatially resolved assemblies of clathrin and caveolin, Rab5a in early endosomes, and a-actinin, often in relationship to cortical actin. In addition, we examined mitochondria, actin, and the Golgi apparatus dynamics in three dimensions. äExtended-resolution structured illumination imaging of endocytic and c(epmc).pdf DeepDyve Pubget Overpricing