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10.1038/srep16895 http://scihub22266oqcxt.onion/10.1038/srep16895 C4655352!4655352 !26592929     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26592929 &cmd=llinks): Failed to open stream: HTTP request failed! 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Biophysical Regulation of Chromatin Architecture Instills a Mechanical Memory in Mesenchymal Stem Cells |pdf=|usr=}}{{26592929 }} gab.com Text Biophysical Regulation of Chromatin Architecture Instills a Mechanical Memory in Mesenchymal Stem Cells JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26592929 #FOAvip Mechanical cues direct the lineage commitment of mesenchymal stem cells (MSCs). In this study, we identified the operative molecular mechanisms through which dynamic tensile loading (DL) regulates changes in chromatin organization and nuclear mechanics in MSCs. Our data show that, in the absence of exogenous differentiation factors, short term DL elicits a rapid increase in chromatin condensation, mediated by acto-myosin based cellular contractility and the activity of the histone-lysine N-methyltransferase EZH2. The resulting change in chromatin condensation stiffened the MSC nucleus, making it less deformable when stretch was applied to the cell. We also identified stretch induced ATP release and purinergic calcium signaling as a central mediator of this chromatin condensation process. Further, we showed that DL, through differential stabilization of the condensed chromatin state, established a 'mechanical memory' in these cells. That is, increasing strain levels and number of loading events led to a greater degree of chromatin condensation that persisted for longer periods of time after the cessation of loading. These data indicate that, with mechanical perturbation, MSCs develop a mechanical memory encoded in structural changes in the nucleus which may sensitize them to future mechanical loading events and define the trajectory and persistence of their lineage specification. Twit Text FOAVip Biophysical Regulation of Chromatin Architecture Instills a Mechanical Memory in Mesenchymal Stem Cells ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26592929 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26592929 #FOAvip English Wikipedia <ref>{{Cite pmid|26592929 }}</ref> Biophysical Regulation of Chromatin Architecture Instills a Mechanical Memory in Mesenchymal Stem Cells #MMPMID26592929 Heo SJ ; Thorpe SD ; Driscoll TP ; Duncan RL ; Lee DA ; Mauck RL Sci Rep 2015[Nov]; 5 (ä): 16895 PMID26592929 show ga Mechanical cues direct the lineage commitment of mesenchymal stem cells (MSCs). In this study, we identified the operative molecular mechanisms through which dynamic tensile loading (DL) regulates changes in chromatin organization and nuclear mechanics in MSCs. Our data show that, in the absence of exogenous differentiation factors, short term DL elicits a rapid increase in chromatin condensation, mediated by acto-myosin based cellular contractility and the activity of the histone-lysine N-methyltransferase EZH2. The resulting change in chromatin condensation stiffened the MSC nucleus, making it less deformable when stretch was applied to the cell. We also identified stretch induced ATP release and purinergic calcium signaling as a central mediator of this chromatin condensation process. Further, we showed that DL, through differential stabilization of the condensed chromatin state, established a 'mechanical memory' in these cells. That is, increasing strain levels and number of loading events led to a greater degree of chromatin condensation that persisted for longer periods of time after the cessation of loading. These data indicate that, with mechanical perturbation, MSCs develop a mechanical memory encoded in structural changes in the nucleus which may sensitize them to future mechanical loading events and define the trajectory and persistence of their lineage specification. |*Mechanotransduction, Cellular [MESH] |Actomyosin/genetics/metabolism [MESH] |Adenosine Triphosphate/metabolism [MESH] |Animals [MESH] |Biomechanical Phenomena [MESH] |Calcium Signaling [MESH] |Calcium/metabolism [MESH] |Cattle [MESH] |Cell Lineage/genetics [MESH] |Cell Nucleus/chemistry/metabolism [MESH] |Chromatin/*metabolism/ultrastructure [MESH] |Cytoskeleton/*metabolism/ultrastructure [MESH] |Gene Expression Regulation [MESH] |Histone-Lysine N-Methyltransferase/*genetics/metabolism [MESH] |Mesenchymal Stem Cells/*metabolism/ultrastructure [MESH] |Stifle/cytology/metabolism [MESH]Biophysical Regulation of Chromatin Architecture Instills a Mechanical(epmc).pdf DeepDyve Pubget Overpricing