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2015 ; 2015
(ä): 196297
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Identification of Anti-Long Chain Saturated Fatty Acid IgG Antibodies in Serum of
Patients with Type 2 Diabetes
#MMPMID26633920
Nicholas DA
; Salto LM
; Boston AM
; Kim NS
; Larios M
; Beeson WL
; Firek AF
; Casiano CA
; Langridge WH
; Cordero-MacIntyre Z
; De Leon M
Mediators Inflamm
2015[]; 2015
(ä): 196297
PMID26633920
show ga
High levels of serum long chain saturated fatty acids (LCSFAs) have been
associated with inflammation in type 2 diabetes. Dietary SFAs can promote
inflammation, the secretion of IgG antibodies, and secretion of the
proinflammatory cytokine IL-1?. This study characterizes anti-LCSFA IgG
antibodies from patients with type 2 diabetes. Serum samples from several cohorts
with type 2 diabetes were analyzed for the presence of anti-LCSFA IgG, the
cytokine IL-1?, and nonesterified fatty acids. Anti-LCSFA IgG was isolated from
patient samples and used for in vitro characterization of avidity and
specificity. A cohort participating in En Balance, a diabetes health education
program that improved diabetes management, tested positive for anti-LCSFA IgG.
Following the 3-month program, the cohort showed a significant reduction in
anti-LCSFA IgG levels. Anti-LCSFA antibodies isolated from these patients
demonstrated high avidity, were specific for long chain SFAs, and correlated with
serum fatty acids in patients with managed type 2 diabetes. Interestingly,
anti-LCSFA IgG neutralized PA-induced IL-1? secretion by dendritic cells. Our
data shows that nonesterified SFAs are recognized by IgG antibodies present in
human blood. The identification of anti-LCSFA IgG antibodies in human sera
establishes a basis for further exploration of lipid induced immune responses in
diabetic patients.