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10.1186/s12951-015-0148-7 http://scihub22266oqcxt.onion/10.1186/s12951-015-0148-7 C4654871!4654871!26589358     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Nanobiotechnology 2015 ; 13 (ä): ä Nephropedia Template TP {{tp|p=26589358|t=2015. Visualization and detection of live and apoptotic cells with fluorescent carbon nanoparticles |pdf=|usr=}}{{26589358}} gab.com Text Visualization and detection of live and apoptotic cells with fluorescent carbon nanoparticles JO: J Nanobiotechnology http://www.kidney.de/pget.php?&tw=1&pmid=26589358 #FOAvip Apoptosis is a genetically encoded cell death program that involves different processes occurring on molecular and sub-cellular levels. Here we report on its new features?the increased accumulation of fluorescent carbon nanoparticles (CDots) in cells and their changed distribution within cell interior, which can witness on altered mechanisms of their translocation through the membrane. The comparative studies of living (intact) and apoptotic cells were provided with two cell lines (HeLa, Vero) using two types of fluorescent nanoparticles (?violet? and ?blue? CDots). In all studied cases the images of living and apoptotic cells were different; the apoptotic cells incorporated larger number of CDots resulting in their much brighter images. These nanoparticles are distributed in cell cytoplasm, however, when the cells are fixed and treated with detergent, their nucleus is also labeled. Flow cytometry allows distinguishing the sub-populations of living and apoptotic cells in their cultures and suggests a very cheap and easy way to characterize them.Electronic supplementary material: The online version of this article (doi:10.1186/s12951-015-0148-7) contains supplementary material, which is available to authorized users. Twit Text FOAVip Visualization and detection of live and apoptotic cells with fluorescent carbon nanoparticles ????J Nanobiotechnology http://www.kidney.de/pget.php?&tw=1&pmid=26589358 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26589358 #FOAvip English Wikipedia <ref>{{Cite pmid|26589358}}</ref> Visualization and detection of live and apoptotic cells with fluorescent carbon nanoparticles #MMPMID26589358 Dekaliuk M; Pyrshev K; Demchenko A J Nanobiotechnology 2015[]; 13 (ä): ä PMID26589358show ga Apoptosis is a genetically encoded cell death program that involves different processes occurring on molecular and sub-cellular levels. Here we report on its new features?the increased accumulation of fluorescent carbon nanoparticles (CDots) in cells and their changed distribution within cell interior, which can witness on altered mechanisms of their translocation through the membrane. The comparative studies of living (intact) and apoptotic cells were provided with two cell lines (HeLa, Vero) using two types of fluorescent nanoparticles (?violet? and ?blue? CDots). In all studied cases the images of living and apoptotic cells were different; the apoptotic cells incorporated larger number of CDots resulting in their much brighter images. These nanoparticles are distributed in cell cytoplasm, however, when the cells are fixed and treated with detergent, their nucleus is also labeled. Flow cytometry allows distinguishing the sub-populations of living and apoptotic cells in their cultures and suggests a very cheap and easy way to characterize them.Electronic supplementary material: The online version of this article (doi:10.1186/s12951-015-0148-7) contains supplementary material, which is available to authorized users. äVisualization and detection of live and apoptotic cells with fluoresce(epmc).pdf DeepDyve Pubget Overpricing