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10.1186/s13075-015-0851-7 http://scihub22266oqcxt.onion/10.1186/s13075-015-0851-7 C4654817!4654817 !26589807     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26589807 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Arthritis+Res+Ther 2015 ; 17 (ä): 337 Nephropedia Template TP {{tp|p=26589807 |t=2015. Peripheral blood natural killer cell percentages in granulomatosis with polyangiitis correlate with disease inactivity and stage |pdf=|usr=}}{{26589807 }} gab.com Text Peripheral blood natural killer cell percentages in granulomatosis with polyangiitis correlate with disease inactivity and stage JO: Arthritis Res Ther http://www.kidney.de/pget.php?&tw=1&pmid=26589807 #FOAvip INTRODUCTION: The role of CD3-CD56+ natural killer (NK) cells in granulomatosis with polyangiitis (GPA) is poorly understood. Recently, it has been shown that peripheral blood NK cells can kill renal microvascular endothelial cells, suggesting a pathogenic role of NK cells in this disease. So far, subset distribution, phenotype, and function of peripheral blood NK cells in relation to GPA disease activity have not been elucidated. Moreover, it is not known whether NK cells infiltrate GPA tissue lesions. METHODS: Paraffin sections of GPA granulomas and controls were stained with anti-CD56 and anti-CD3 antibodies. Peripheral blood lymphocyte subsets were analyzed by flow cytometry. NK cell degranulation was analyzed using cocultures of patient PBMCs with target cells and surface expression of CD107a. Clinical data were extracted from medical records. Statistical analysis was performed in an exploratory way. RESULTS: CD56+ cells were not detectable in active granulomatous GPA lesions but were found frequently in granulomas from tuberculosis and sarcoidosis patients. In GPA, the proportion of NK cells among peripheral blood lymphocytes correlated negatively with the Birmingham Vasculitis Activity Score (BVAS) (n?=?28). Accordingly, NK cell percentages correlated positively with the duration of remission (n?=?28) and were significantly higher in inactive GPA (BVAS?=?0, n?=?17) than in active GPA, healthy controls (n?=?29), and inactive control diseases (n?=?12). The highest NK cell percentages were found in patients with long-term remission and tapered immunosuppressive therapy. NK cell percentages >18.5% of peripheral blood lymphocytes (n?=?12/28) determined GPA inactivity with a specificity of 100%. The differentiation into CD56(dim) and CD56(bright) NK cell subsets was unchanged in GPA (n?=?28), irrespective of disease activity. Similar surface expression of the activating NK cell-receptors (NKp30, NKp46, and NKG2D) was determined. Like in healthy controls, GPA NK cells degranulated in the presence of NK cell receptor ligand bearing epithelial and lymphatic target cells. CONCLUSIONS: NK cells were not detectable in GPA granulomas. Peripheral blood NK cell percentages positively correlate with the suppression of GPA activity and could serve as a biomarker for GPA activity. Peripheral blood NK cells in GPA patients are mature NK cells with preserved immune recognition. Twit Text FOAVip Peripheral blood natural killer cell percentages in granulomatosis with polyangiitis correlate with disease inactivity and stage ????Arthritis Res Ther http://www.kidney.de/pget.php?&tw=1&pmid=26589807 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26589807 #FOAvip English Wikipedia <ref>{{Cite pmid|26589807 }}</ref> Peripheral blood natural killer cell percentages in granulomatosis with polyangiitis correlate with disease inactivity and stage #MMPMID26589807 Merkt W ; Sturm P ; Lasitschka F ; Tretter T ; Watzl C ; Saure D ; Hundemer M ; Schwenger V ; Blank N ; Lorenz HM ; Cerwenka A Arthritis Res Ther 2015[Nov]; 17 (ä): 337 PMID26589807 show ga INTRODUCTION: The role of CD3-CD56+ natural killer (NK) cells in granulomatosis with polyangiitis (GPA) is poorly understood. Recently, it has been shown that peripheral blood NK cells can kill renal microvascular endothelial cells, suggesting a pathogenic role of NK cells in this disease. So far, subset distribution, phenotype, and function of peripheral blood NK cells in relation to GPA disease activity have not been elucidated. Moreover, it is not known whether NK cells infiltrate GPA tissue lesions. METHODS: Paraffin sections of GPA granulomas and controls were stained with anti-CD56 and anti-CD3 antibodies. Peripheral blood lymphocyte subsets were analyzed by flow cytometry. NK cell degranulation was analyzed using cocultures of patient PBMCs with target cells and surface expression of CD107a. Clinical data were extracted from medical records. Statistical analysis was performed in an exploratory way. RESULTS: CD56+ cells were not detectable in active granulomatous GPA lesions but were found frequently in granulomas from tuberculosis and sarcoidosis patients. In GPA, the proportion of NK cells among peripheral blood lymphocytes correlated negatively with the Birmingham Vasculitis Activity Score (BVAS) (n?=?28). Accordingly, NK cell percentages correlated positively with the duration of remission (n?=?28) and were significantly higher in inactive GPA (BVAS?=?0, n?=?17) than in active GPA, healthy controls (n?=?29), and inactive control diseases (n?=?12). The highest NK cell percentages were found in patients with long-term remission and tapered immunosuppressive therapy. NK cell percentages >18.5% of peripheral blood lymphocytes (n?=?12/28) determined GPA inactivity with a specificity of 100%. The differentiation into CD56(dim) and CD56(bright) NK cell subsets was unchanged in GPA (n?=?28), irrespective of disease activity. Similar surface expression of the activating NK cell-receptors (NKp30, NKp46, and NKG2D) was determined. Like in healthy controls, GPA NK cells degranulated in the presence of NK cell receptor ligand bearing epithelial and lymphatic target cells. CONCLUSIONS: NK cells were not detectable in GPA granulomas. Peripheral blood NK cell percentages positively correlate with the suppression of GPA activity and could serve as a biomarker for GPA activity. Peripheral blood NK cells in GPA patients are mature NK cells with preserved immune recognition. |Adult [MESH] |Aged [MESH] |Aged, 80 and over [MESH] |Cells, Cultured [MESH] |Disease Progression [MESH] |Female [MESH] |Granulomatosis with Polyangiitis/*metabolism/*pathology [MESH] |Humans [MESH] |Killer Cells, Natural/*metabolism/*pathology [MESH] |Leukocytes, Mononuclear/*metabolism/*pathology [MESH] |Male [MESH]Peripheral blood natural killer cell percentages in granulomatosis wit(epmc).pdf DeepDyve Pubget Overpricing