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10.1186/s13000-015-0435-5 http://scihub22266oqcxt.onion/10.1186/s13000-015-0435-5 C4650491!4650491!26576674     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Diagn+Pathol 2015 ; 10 (ä): ä Nephropedia Template TP {{tp|p=26576674|t=2015. miR-449a targets Flot2 and inhibits gastric cancer invasion by inhibiting TGF-?-mediated EMT |pdf=|usr=}}{{26576674}} gab.com Text miR-449a targets Flot2 and inhibits gastric cancer invasion by inhibiting TGF- -mediated EMT JO: Diagn Pathol http://www.kidney.de/pget.php?&tw=1&pmid=26576674 #FOAvip Background: Flot2, a highly conserved protein of the SPFH domain containing proteins family, has recently been identified as oncogene to be involved in the tumorigenesis and metastasis of several cancers including gastric cancer. However, the underlying molecular mechanism of Flot2 in gastric cancer (GC) is largely unknown. Methods: qRT-PCR and western blot was performed to detect miR-449a and Flot2 expression in GC cell lines and Normal human gastric epithelial cells. Then, luciferase reporter assay was used to elucidate whether Flot2 is a target gene of miR-449a. Finally, the roles and mechanism of miR-449a in regulation of tumor invasion were further investigated. Results: In this study, miR-449a expression was downregulated and Flot2 was upregulated in all GC cell lines as compared with that in GES-1. luciferase reporter assay identified Flot2 as a novel direct target of miR-449a. miR-449a regulated GC cell invasion by suppressing Flot2 expression. Expression analysis of a set of epithelial-mesenchymal transition (EMT) markers showed that miR-449a reduced the expression of mesenchymal markers (vimentin and N-cadherin) and induced the expression of epithelial marker (E-cadherin), which was consistent with silenced Flot2. Moreover, Flot2 is necessary for TGF-?-induced EMT in GC cells. Conclusions: Our results demonstrated that miR-449a suppressed Flot2 expression results in decreased cell invasion through repressing TGF-?-mediated-EMT, and provides a new theoretical basis to further investigate miR-449a-regulated Flot2 as a potential biomarker and a promising approach for GC treatment. Twit Text FOAVip miR-449a targets Flot2 and inhibits gastric cancer invasion by inhibiting TGF- -mediated EMT ????Diagn Pathol http://www.kidney.de/pget.php?&tw=1&pmid=26576674 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26576674 #FOAvip English Wikipedia <ref>{{Cite pmid|26576674}}</ref> miR-449a targets Flot2 and inhibits gastric cancer invasion by inhibiting TGF-?-mediated EMT #MMPMID26576674 Li Q; Peng J; Li X; Leng A; Liu T Diagn Pathol 2015[]; 10 (ä): ä PMID26576674show ga Background: Flot2, a highly conserved protein of the SPFH domain containing proteins family, has recently been identified as oncogene to be involved in the tumorigenesis and metastasis of several cancers including gastric cancer. However, the underlying molecular mechanism of Flot2 in gastric cancer (GC) is largely unknown. Methods: qRT-PCR and western blot was performed to detect miR-449a and Flot2 expression in GC cell lines and Normal human gastric epithelial cells. Then, luciferase reporter assay was used to elucidate whether Flot2 is a target gene of miR-449a. Finally, the roles and mechanism of miR-449a in regulation of tumor invasion were further investigated. Results: In this study, miR-449a expression was downregulated and Flot2 was upregulated in all GC cell lines as compared with that in GES-1. luciferase reporter assay identified Flot2 as a novel direct target of miR-449a. miR-449a regulated GC cell invasion by suppressing Flot2 expression. Expression analysis of a set of epithelial-mesenchymal transition (EMT) markers showed that miR-449a reduced the expression of mesenchymal markers (vimentin and N-cadherin) and induced the expression of epithelial marker (E-cadherin), which was consistent with silenced Flot2. Moreover, Flot2 is necessary for TGF-?-induced EMT in GC cells. Conclusions: Our results demonstrated that miR-449a suppressed Flot2 expression results in decreased cell invasion through repressing TGF-?-mediated-EMT, and provides a new theoretical basis to further investigate miR-449a-regulated Flot2 as a potential biomarker and a promising approach for GC treatment. ämiR-449a targets Flot2 and inhibits gastric cancer invasion by inhibit(epmc).pdf DeepDyve Pubget Overpricing