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10.1038/srep10833 http://scihub22266oqcxt.onion/10.1038/srep10833 C4649911!4649911!26035283     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2015 ; 5 (ä): ä Nephropedia Template TP {{tp|p=26035283|t=2015. CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus |pdf=|usr=}}{{26035283}} gab.com Text CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26035283 #FOAvip Chronic hepatitis B virus (HBV) infection is prevalent, deadly, and seldom cured due to the persistence of viral episomal DNA (cccDNA) in infected cells. Newly developed genome engineering tools may offer the ability to directly cleave viral DNA, thereby promoting viral clearance. Here, we show that the CRISPR/Cas9 system can specifically target and cleave conserved regions in the HBV genome, resulting in robust suppression of viral gene expression and replication. Upon sustained expression of Cas9 and appropriately chosen guide RNAs, we demonstrate cleavage of cccDNA by Cas9 and a dramatic reduction in both cccDNA and other parameters of viral gene expression and replication. Thus, we show that directly targeting viral episomal DNA is a novel therapeutic approach to control the virus and possibly cure patients. Twit Text FOAVip CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26035283 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26035283 #FOAvip English Wikipedia <ref>{{Cite pmid|26035283}}</ref> CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus #MMPMID26035283 Ramanan V; Shlomai A; Cox DB; Schwartz RE; Michailidis E; Bhatta A; Scott DA; Zhang F; Rice CM; Bhatia SN Sci Rep 2015[]; 5 (ä): ä PMID26035283show ga Chronic hepatitis B virus (HBV) infection is prevalent, deadly, and seldom cured due to the persistence of viral episomal DNA (cccDNA) in infected cells. Newly developed genome engineering tools may offer the ability to directly cleave viral DNA, thereby promoting viral clearance. Here, we show that the CRISPR/Cas9 system can specifically target and cleave conserved regions in the HBV genome, resulting in robust suppression of viral gene expression and replication. Upon sustained expression of Cas9 and appropriately chosen guide RNAs, we demonstrate cleavage of cccDNA by Cas9 and a dramatic reduction in both cccDNA and other parameters of viral gene expression and replication. Thus, we show that directly targeting viral episomal DNA is a novel therapeutic approach to control the virus and possibly cure patients. äCRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B v(epmc).pdf DeepDyve Pubget Overpricing