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10.1534/genetics.115.180208 http://scihub22266oqcxt.onion/10.1534/genetics.115.180208 C4649654!4649654!26320097     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Genetics 2015 ; 201 (3): 843-52 Nephropedia Template TP {{tp|p=26320097|t=2015. The Transgenic RNAi Project at Harvard Medical School: Resources and Validation |pdf=|usr=}}{{26320097}} gab.com Text The Transgenic RNAi Project at Harvard Medical School: Resources and Validation JO: Genetics http://www.kidney.de/pget.php?&tw=1&pmid=26320097 #FOAvip To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT?qPCR and phenotypic analyses, and developing tools and web resources for identifying RNAi lines and retrieving existing information on their quality. With these goals in mind, here we describe in detail the various tools we developed and the status of the collection, which is currently composed of 11,491 lines and covering 71% of Drosophila genes. Data on the characterization of the lines either by RT?qPCR or phenotype is available on a dedicated website, the RNAi Stock Validation and Phenotypes Project (RSVP, http://www.flyrnai.org/RSVP.html), and stocks are available from three stock centers, the Bloomington Drosophila Stock Center (United States), National Institute of Genetics (Japan), and TsingHua Fly Center (China). Twit Text FOAVip The Transgenic RNAi Project at Harvard Medical School: Resources and Validation ????Genetics http://www.kidney.de/pget.php?&tw=1&pmid=26320097 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26320097 #FOAvip English Wikipedia <ref>{{Cite pmid|26320097}}</ref> The Transgenic RNAi Project at Harvard Medical School: Resources and Validation #MMPMID26320097 Perkins LA; Holderbaum L; Tao R; Hu Y; Sopko R; McCall K; Yang-Zhou D; Flockhart I; Binari R; Shim HS; Miller A; Housden A; Foos M; Randkelv S; Kelley C; Namgyal P; Villalta C; Liu LP; Jiang X; Huan-Huan Q; Wang X; Fujiyama A; Toyoda A; Ayers K; Blum A; Czech B; Neumuller R; Yan D; Cavallaro A; Hibbard K; Hall D; Cooley L; Hannon GJ; Lehmann R; Parks A; Mohr SE; Ueda R; Kondo S; Ni JQ; Perrimon N Genetics 2015[Nov]; 201 (3): 843-52 PMID26320097show ga To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT?qPCR and phenotypic analyses, and developing tools and web resources for identifying RNAi lines and retrieving existing information on their quality. With these goals in mind, here we describe in detail the various tools we developed and the status of the collection, which is currently composed of 11,491 lines and covering 71% of Drosophila genes. Data on the characterization of the lines either by RT?qPCR or phenotype is available on a dedicated website, the RNAi Stock Validation and Phenotypes Project (RSVP, http://www.flyrnai.org/RSVP.html), and stocks are available from three stock centers, the Bloomington Drosophila Stock Center (United States), National Institute of Genetics (Japan), and TsingHua Fly Center (China). äThe Transgenic RNAi Project at Harvard Medical School: Resources and V(epmc).pdf DeepDyve Pubget Overpricing