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Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Sci+Rep 2015 ; 5 (ä): ä Nephropedia Template TP
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Peritoneal VEGF-A expression is regulated by TGF-?1 through an ID1 pathway in women with endometriosis #MMPMID26577912
Young VJ; Ahmad SF; Brown JK; Duncan WC; Horne AW
Sci Rep 2015[]; 5 (ä): ä PMID26577912show ga
VEGF-A, an angiogenic factor, is increased in the peritoneal fluid of women with endometriosis. The cytokine TGF-?1 is thought to play a role in the establishment of endometriosis lesions. Inhibitor of DNA binding (ID) proteins are transcriptional targets of TGF-?1 and ID1 has been implicated in VEGF-A regulation during tumor angiogenesis. Herein, we determined whether peritoneal expression of VEGF-A is regulated by TGF-?1 through the ID1 pathway in women with endometriosis. VEGF-A was measured in peritoneal fluid by ELISA (n?=?16). VEGF-A and ID1 expression was examined in peritoneal biopsies (n?=?13), and primary peritoneal and immortalized mesothelial cells (MeT5A) by immunohistochemistry, qRT-PCR and ELISA. VEGF-A was increased in peritoneal fluid from women with endometriosis and levels correlated with TGF-?1 concentrations (P?0.05). VEGF-A was immunolocalized to peritoneal mesothelium and TGF-?1 increased VEGFA mRNA (P?0.05) and protein (P?0.05) in mesothelial cells. ID1 was increased in peritoneum from women with endometriosis and TGF-?1 increased concentrations of ID1 mRNA (P?0.05) in mesothelial cells. VEGF-A regulation through ID1 was confirmed by siRNA in MeT5A cells (P?0.05). Our data supports role for ID1 in the pathophysiology of endometriosis, as an effector of TGF?1 dependent upregulation of VEGF-A, and highlights a novel potential therapeutic target.