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2015 ; 135
(12
): 2944-2954
Nephropedia Template TP
gab.com Text
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English Wikipedia
MCPIP1 Regulates Fibroblast Migration in 3-D Collagen Matrices Downstream of MAP
Kinases and NF-?B
#MMPMID26399696
Chao J
; Dai X
; Peņa T
; Doyle DA
; Guenther TM
; Carlson MA
J Invest Dermatol
2015[Dec]; 135
(12
): 2944-2954
PMID26399696
show ga
The fibroblast-populated three-dimensional (3-D) collagen matrix has been used to
model matrix contraction, cell motility, and general fibroblast biology. MCPIP1
(monocyte chemotactic protein-induced protein 1) has been shown to regulate
inflammation, angiogenesis, and cellular motility. In the present study, we
demonstrated induction of MCPIP1 in human fibroblasts embedded in the
stress-released 3-D collagen matrix, which occurred through activation of
mitogen-activated protein kinases, phosphoinositide 3-kinase, and NF-?B.
Furthermore, MCPIP1 induction was associated with inhibition of fibroblast
migration out of the nested collagen matrix. MCPIP1 induction or ectopic
expression also upregulated p53. RNA interference of p53 prevented the inhibition
of migration produced by induction or ectopic expression of MCPIP1. Our findings
suggest a new role for MCPIP1 as a molecular switch that regulates fibroblast
migration in the nested collagen matrix model.
|Cell Movement
[MESH]
|Cells, Cultured
[MESH]
|Collagen/*metabolism
[MESH]
|Fibroblasts/*physiology
[MESH]
|Humans
[MESH]
|Mitogen-Activated Protein Kinases/*physiology
[MESH]