A ChIP on the shoulder? Chromatin immunoprecipitation and validation strategies
for ChIP antibodies
#MMPMID26594335
Wardle FC
; Tan H
F1000Res
2015[]; 4
(?): 235
PMID26594335
show ga
Chromatin immunoprecipitation (ChIP) is a technique widely used in the study of
epigenetics and transcriptional regulation of gene expression. However, its
antibody-centric nature exposes it to similar challenges faced by other
antibody-based procedures, of which the most prominent are issues of specificity
and affinity in antigen recognition. As with other techniques that make use of
antibodies, recent studies have shown the need for validation of ChIP antibodies
in order to be sure they recognize the advertised protein or epitope. We
summarize here the issues surrounding ChIP antibody usage, and highlight the
toolkit of validation methods that can be employed by investigators looking to
appraise these reagents.
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