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10.1074/jbc.M115.681205

http://scihub22266oqcxt.onion/10.1074/jbc.M115.681205
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C4646914!4646914!26405032
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suck abstract from ncbi


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pmid26405032      J+Biol+Chem 2015 ; 290 (46): 27712-22
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  • Assembling a Correctly Folded and Functional Heptahelical Membrane Protein by Protein Trans-splicing* #MMPMID26405032
  • Mehler M; Eckert CE; Busche A; Kulhei J; Michaelis J; Becker-Baldus J; Wachtveitl J; Dötsch V; Glaubitz C
  • J Biol Chem 2015[Nov]; 290 (46): 27712-22 PMID26405032show ga
  • Background: Protein trans-splicing as a molecular design tool has been demonstrated for soluble but not yet for membrane proteins.Results: Two separate polypeptides have been spliced in vivo, yielding correctly folded and functional proteorhodopsin.Conclusion:Trans-splicing of ?-helical membrane proteins under native conditions is possible.Significance: Our findings are important for the folding, assembly, and engineering of membrane proteins.
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