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2015 ; 44
(8
): 1121-5
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Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological,
Pathological and Molecular Methods
#MMPMID26587476
Masoumi O
; Shahzadi M
; Kordbacheh P
; Zaini F
; Mahmoudi S
; Mahmoudi M
; Bahreini H
; Safara M
; Mirhendi H
Iran J Public Health
2015[Aug]; 44
(8
): 1121-5
PMID26587476
show ga
BACKGROUND: The aim of this study was to detect fungi in atherosclerotic plaques
and investigate their possible role in atherosclerosis. METHODS: Coronary
atherosclerotic plaques specimen were obtained from patients with
atherosclerosis. Direct examination, culture, histopathology study, PCR and
sequencing were performed to detect/identify the mycotic elements in the plaques.
Age, sex, smoking, obesity, hypertension, hyperlipidemia, family history of heart
diseases and diabetes were considered and data were analyzed using Chi Square
test by SPSS version 15. RESULTS: A total of 41 specimens were analyzed. Direct
examination for fungal elements was negative in all cases but in culture only one
specimen grew as a mold colony. The presence of fungal elements were confirmed in
6 and 2 tissue sections stained by Gomori methenamine silver and Hematoxylin and
Eosin methods, respectively. Using PCR, 11 cases were positive for fungi. The DNA
sequence analysis of six positive specimens which were randomly selected revealed
fungi as Candida albicans (n=3), Candida guilliermondii (n=2) and Monilia sp.
(n=1). CONCLUSION: A significant association between the presence of fungi in
atherosclerotic plaques and severity of atherogenesis and atherosclerotic disease
was not found. This could be due to limited numbers of patients included in our
study. However, the presence of fungal elements in 26.8% of our specimens is
considerable and the results does not exclude the correlation between the
presence of fungi with atherosclerosis and coronary artery disease.