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Characterization and quantification of proteins secreted by single human embryos
prior to implantation
#MMPMID26471863
Poli M
; Ori A
; Child T
; Jaroudi S
; Spath K
; Beck M
; Wells D
EMBO Mol Med
2015[Nov]; 7
(11
): 1465-79
PMID26471863
show ga
The use of in vitro fertilization (IVF) has revolutionized the treatment of
infertility and is now responsible for 1-5% of all births in industrialized
countries. During IVF, it is typical for patients to generate multiple embryos.
However, only a small proportion of them possess the genetic and metabolic
requirements needed in order to produce a healthy pregnancy. The identification
of the embryo with the greatest developmental capacity represents a major
challenge for fertility clinics. Current methods for the assessment of embryo
competence are proven inefficient, and the inadvertent transfer of non-viable
embryos is the principal reason why most IVF treatments (approximately
two-thirds) end in failure. In this study, we investigate how the application of
proteomic measurements could improve success rates in clinical embryology. We
describe a procedure that allows the identification and quantification of
proteins of embryonic origin, present in attomole concentrations in the
blastocoel, the enclosed fluid-filled cavity that forms within 5-day-old human
embryos. By using targeted proteomics, we demonstrate the feasibility of
quantifying multiple proteins in samples derived from single blastocoels and that
such measurements correlate with aspects of embryo viability, such as chromosomal
(ploidy) status. This study illustrates the potential of high-sensitivity
proteomics to measure clinically relevant biomarkers in minute samples and, more
specifically, suggests that key aspects of embryo competence could be measured
using a proteomic-based strategy, with negligible risk of harm to the living
embryo. Our work paves the way for the development of "next-generation" embryo
competence assessment strategies, based on functional proteomics.
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