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10.1371/journal.pone.0138423 http://scihub22266oqcxt.onion/10.1371/journal.pone.0138423 C4643060!4643060!26562662     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26562662.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2015 ; 10 (11): ä Nephropedia Template TP {{tp|p=26562662|t=2015. Interleukin (IL)-1? Is a Strong Inducer of IL-36? Expression in Human Colonic Myofibroblasts |pdf=|usr=}}{{26562662}} gab.com Text Interleukin (IL)-1 Is a Strong Inducer of IL-36 Expression in Human Colonic Myofibroblasts JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26562662 #FOAvip Backgrounds and aims: Interleukin (IL)-36 cytokines are members of the IL-1 cytokine family. In this study, we investigated the expression of IL-36? in human colonic myofibroblasts to explore the molecular mechanisms underlying IL-36? induction. Materials and methods: IL-36 mRNA was analyzed by real-time PCR method. Secretion of IL-36? protein was evaluated by Western blot and ELISA analyses. Molecular mechanism of IL-36? induction was evaluated by siRNA analyses and immunofluorescence experiments. Results: IL-36? mRNA expression was scarcely detected in the cells without stimulation. IL-1? induced a marked increase of IL-36? mRNA expression. TNF-? markedly enhanced IL-1?-induced IL-36? mRNA expression. These responses were confirmed at the protein levels. The inhibitors for ERK1/2 (PD98059 and U0216) and a p38 MAPK (SB203580) significantly reduced the IL-1?-induced IL-36? mRNA expression. In addition, the siRNAs specific for NF-?B p65 and AP-1 (c-Jun) significantly reduced the expression of IL-1?-induced IL-36? mRNA. Conclusions: Colonic myofibroblasts are cellular source of IL-36? in the intestine. IL-36? expression was induced by the combination of IL-1? and TNF-? via activation of MAPKs and transcription factors, NF-?B and AP-1. Twit Text FOAVip Interleukin (IL)-1 Is a Strong Inducer of IL-36 Expression in Human Colonic Myofibroblasts ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26562662 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26562662 #FOAvip English Wikipedia <ref>{{Cite pmid|26562662}}</ref> Interleukin (IL)-1? Is a Strong Inducer of IL-36? Expression in Human Colonic Myofibroblasts #MMPMID26562662 Takahashi K; Nishida A; Shioya M; Imaeda H; Bamba S; Inatomi O; Shimizu T; Kitoh K; Andoh A PLoS One 2015[]; 10 (11): ä PMID26562662show ga Backgrounds and aims: Interleukin (IL)-36 cytokines are members of the IL-1 cytokine family. In this study, we investigated the expression of IL-36? in human colonic myofibroblasts to explore the molecular mechanisms underlying IL-36? induction. Materials and methods: IL-36 mRNA was analyzed by real-time PCR method. Secretion of IL-36? protein was evaluated by Western blot and ELISA analyses. Molecular mechanism of IL-36? induction was evaluated by siRNA analyses and immunofluorescence experiments. Results: IL-36? mRNA expression was scarcely detected in the cells without stimulation. IL-1? induced a marked increase of IL-36? mRNA expression. TNF-? markedly enhanced IL-1?-induced IL-36? mRNA expression. These responses were confirmed at the protein levels. The inhibitors for ERK1/2 (PD98059 and U0216) and a p38 MAPK (SB203580) significantly reduced the IL-1?-induced IL-36? mRNA expression. In addition, the siRNAs specific for NF-?B p65 and AP-1 (c-Jun) significantly reduced the expression of IL-1?-induced IL-36? mRNA. Conclusions: Colonic myofibroblasts are cellular source of IL-36? in the intestine. IL-36? expression was induced by the combination of IL-1? and TNF-? via activation of MAPKs and transcription factors, NF-?B and AP-1. äInterleukin (IL)-1? Is a Strong Inducer of IL-36? Expression in Human (epmc).pdf DeepDyve Pubget Overpricing