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10.1038/srep16552 http://scihub22266oqcxt.onion/10.1038/srep16552 C4642271!4642271 !26559755     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26559755 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2015 ; 5 (ä): 16552 Nephropedia Template TP {{tp|p=26559755 |t=2015. Activation of TGF-?1-CD147 positive feedback loop in hepatic stellate cells promotes liver fibrosis |pdf=|usr=}}{{26559755 }} gab.com Text Activation of TGF- 1-CD147 positive feedback loop in hepatic stellate cells promotes liver fibrosis JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26559755 #FOAvip Activation of hepatic stellate cells (HSCs) by transforming growth factor-?1 (TGF-?1) initiates HBV-associated fibrogenesis. The mechanism of TGF-?1 modulating HSC activation is not fully uncovered. We hypothesized a positive feedback signaling loop of TGF-?1-CD147 promoting liver fibrogenesis by activation of HSCs. Human HSC cell line LX-2 and spontaneous liver fibrosis model derived from HBV transgenic mice were used to evaluate the activation of molecules in the signaling loop. Wound healing and cell contraction assay were performed to detect the CD147-overexpressed HSC migration and contraction. The transcriptional regulation of CD147 by TGF-?1/Smad4 was determined using dual-luciferase reporter assay and chromatin immunoprecipitation. We found that a positive reciprocal regulation between TGF-?1 and CD147 mediated HSC activation. CD147 over-expression promoted HSC migration and accelerated TGF-?1-induced cell contraction. Phosphorylation of Smad2 and Smad3 in cooperation with Smad4 mediated the TGF-?1-regulated CD147 expression. Smad4 activated the transcription by direct interaction with CD147 promoter. Meanwhile, CD147 modulated the activated phenotype of HSCs through the ERK1/2 and Sp1 which up-regulated ?-SMA, collagen I, and TGF-?1 synthesis. These findings indicate that TGF-?1-CD147 loop plays a key role in regulating the HSC activation and combination of TGF-? receptor inhibitor and anti-CD147 antibody might be promised to reverse fibrogenesis. Twit Text FOAVip Activation of TGF- 1-CD147 positive feedback loop in hepatic stellate cells promotes liver fibrosis ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26559755 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26559755 #FOAvip English Wikipedia <ref>{{Cite pmid|26559755 }}</ref> Activation of TGF-?1-CD147 positive feedback loop in hepatic stellate cells promotes liver fibrosis #MMPMID26559755 Li HY ; Ju D ; Zhang DW ; Li H ; Kong LM ; Guo Y ; Li C ; Wang XL ; Chen ZN ; Bian H Sci Rep 2015[Nov]; 5 (ä): 16552 PMID26559755 show ga Activation of hepatic stellate cells (HSCs) by transforming growth factor-?1 (TGF-?1) initiates HBV-associated fibrogenesis. The mechanism of TGF-?1 modulating HSC activation is not fully uncovered. We hypothesized a positive feedback signaling loop of TGF-?1-CD147 promoting liver fibrogenesis by activation of HSCs. Human HSC cell line LX-2 and spontaneous liver fibrosis model derived from HBV transgenic mice were used to evaluate the activation of molecules in the signaling loop. Wound healing and cell contraction assay were performed to detect the CD147-overexpressed HSC migration and contraction. The transcriptional regulation of CD147 by TGF-?1/Smad4 was determined using dual-luciferase reporter assay and chromatin immunoprecipitation. We found that a positive reciprocal regulation between TGF-?1 and CD147 mediated HSC activation. CD147 over-expression promoted HSC migration and accelerated TGF-?1-induced cell contraction. Phosphorylation of Smad2 and Smad3 in cooperation with Smad4 mediated the TGF-?1-regulated CD147 expression. Smad4 activated the transcription by direct interaction with CD147 promoter. Meanwhile, CD147 modulated the activated phenotype of HSCs through the ERK1/2 and Sp1 which up-regulated ?-SMA, collagen I, and TGF-?1 synthesis. These findings indicate that TGF-?1-CD147 loop plays a key role in regulating the HSC activation and combination of TGF-? receptor inhibitor and anti-CD147 antibody might be promised to reverse fibrogenesis. |Animals [MESH] |Basigin/genetics/*metabolism [MESH] |Cell Line [MESH] |Cell Transdifferentiation/genetics [MESH] |Collagen Type I/genetics/metabolism [MESH] |Gene Expression [MESH] |Gene Expression Regulation [MESH] |Hepatic Stellate Cells/cytology/*metabolism [MESH] |Humans [MESH] |Liver Cirrhosis/etiology/*metabolism/*pathology [MESH] |Mice [MESH] |Mice, Transgenic [MESH] |Mitogen-Activated Protein Kinase 1/metabolism [MESH] |Mitogen-Activated Protein Kinase 3/metabolism [MESH] |Promoter Regions, Genetic [MESH] |Protein Binding [MESH] |Signal Transduction [MESH] |Smad Proteins/metabolism [MESH] |Smad4 Protein/metabolism [MESH] |Sp1 Transcription Factor/metabolism [MESH]Activation of TGF-?1-CD147 positive feedback loop in hepatic stellate (epmc).pdf DeepDyve Pubget Overpricing