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10.1186/s12958-015-0118-9 http://scihub22266oqcxt.onion/10.1186/s12958-015-0118-9 C4640411!4640411 !26553294     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26553294 &cmd=llinks): Failed to open stream: HTTP request failed! 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Metabolites involved in cellular communication among human cumulus-oocyte-complex and sperm during in vitro fertilization |pdf=|usr=}}{{26553294 }} gab.com Text Metabolites involved in cellular communication among human cumulus-oocyte-complex and sperm during in vitro fertilization JO: Reprod Biol Endocrinol http://www.kidney.de/pget.php?&tw=1&pmid=26553294 #FOAvip BACKGROUND: Fertilization is a key physiological process for the preservation of the species. Consequently, different mechanisms affecting the sperm and the oocyte have been developed to ensure a successful fertilization. Thus, sperm acrosome reaction is necessary for the egg coat penetration and sperm-oolema fusion. Several molecules are able to induce the sperm acrosome reaction; however, this process should be produced coordinately in time and in the space to allow the success of fertilization between gametes. The goal of this study was to analyze the metabolites secreted by cumulus-oocyte-complex (COC) to find out new components that could contribute to the induction of the human sperm acrosome reaction and other physiological processes at the time of gamete interaction and fertilization. METHODS: For the metabolomic analysis, eighteen aliquots of medium were used in each group, containing: a) only COC before insemination and after 3 h of incubation; b) COC and capacitated spermatozoa after insemination and incubated for 16-20 hours; c) only capacitated sperm after 16-20 h in culture and d) only fertilization medium as control. Six patients undergoing assisted reproduction whose male partners provided normozoospermic samples were included in the study. Seventy-two COC were inseminated. RESULTS: The metabolites identified were monoacylglycerol (MAG), lysophosphatidylcholine (LPC) and phytosphingosine (PHS). Analysis by PCR and in silico of the gene expression strongly suggests that the cumulus cells contribute to the formation of the PHS and LPC. CONCLUSIONS: LPC and PHS are secreted by cumulus cells during in vitro fertilization and they could be involved in the induction of human acrosome reaction (AR). The identification of new molecules with a paracrine effect on oocytes, cumulus cells and spermatozoa will provide a better understanding of gamete interaction. Twit Text FOAVip Metabolites involved in cellular communication among human cumulus-oocyte-complex and sperm during in vitro fertilization ????Reprod Biol Endocrinol http://www.kidney.de/pget.php?&tw=1&pmid=26553294 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26553294 #FOAvip English Wikipedia <ref>{{Cite pmid|26553294 }}</ref> Metabolites involved in cellular communication among human cumulus-oocyte-complex and sperm during in vitro fertilization #MMPMID26553294 Gómez-Torres MJ ; García EM ; Guerrero J ; Medina S ; Izquierdo-Rico MJ ; Gil-Izquierdo Á ; Orduna J ; Savirón M ; González-Brusi L ; Ten J ; Bernabeu R ; Avilés M Reprod Biol Endocrinol 2015[Nov]; 13 (ä): 123 PMID26553294 show ga BACKGROUND: Fertilization is a key physiological process for the preservation of the species. Consequently, different mechanisms affecting the sperm and the oocyte have been developed to ensure a successful fertilization. Thus, sperm acrosome reaction is necessary for the egg coat penetration and sperm-oolema fusion. Several molecules are able to induce the sperm acrosome reaction; however, this process should be produced coordinately in time and in the space to allow the success of fertilization between gametes. The goal of this study was to analyze the metabolites secreted by cumulus-oocyte-complex (COC) to find out new components that could contribute to the induction of the human sperm acrosome reaction and other physiological processes at the time of gamete interaction and fertilization. METHODS: For the metabolomic analysis, eighteen aliquots of medium were used in each group, containing: a) only COC before insemination and after 3 h of incubation; b) COC and capacitated spermatozoa after insemination and incubated for 16-20 hours; c) only capacitated sperm after 16-20 h in culture and d) only fertilization medium as control. Six patients undergoing assisted reproduction whose male partners provided normozoospermic samples were included in the study. Seventy-two COC were inseminated. RESULTS: The metabolites identified were monoacylglycerol (MAG), lysophosphatidylcholine (LPC) and phytosphingosine (PHS). Analysis by PCR and in silico of the gene expression strongly suggests that the cumulus cells contribute to the formation of the PHS and LPC. CONCLUSIONS: LPC and PHS are secreted by cumulus cells during in vitro fertilization and they could be involved in the induction of human acrosome reaction (AR). The identification of new molecules with a paracrine effect on oocytes, cumulus cells and spermatozoa will provide a better understanding of gamete interaction. |Acrosome Reaction/physiology [MESH] |Cell Communication/*physiology [MESH] |Cumulus Cells/cytology/*metabolism [MESH] |Female [MESH] |Fertilization in Vitro [MESH] |Humans [MESH] |Lysophosphatidylcholines/metabolism [MESH] |Male [MESH] |Monoglycerides/metabolism [MESH] |Oocytes/cytology/*metabolism [MESH] |Sperm Capacitation/physiology [MESH] |Sperm-Ovum Interactions/*physiology [MESH] |Spermatozoa/cytology/*metabolism [MESH]Metabolites involved in cellular communication among human cumulus-ooc(epmc).pdf DeepDyve Pubget Overpricing