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10.1038/jcbfm.2015.26

http://scihub22266oqcxt.onion/10.1038/jcbfm.2015.26
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C4640252!4640252 !25712498
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suck abstract from ncbi


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pmid25712498
      J+Cereb+Blood+Flow+Metab 2015 ; 35 (6 ): 933-42
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  • Chronic monitoring of vascular progression after ischemic stroke using multiexposure speckle imaging and two-photon fluorescence microscopy #MMPMID25712498
  • Schrandt CJ ; Kazmi SM ; Jones TA ; Dunn AK
  • J Cereb Blood Flow Metab 2015[Jun]; 35 (6 ): 933-42 PMID25712498 show ga
  • Monitoring the progression of the vascular structure and cerebral blood flow (CBF) after brain injury is vital to understand the neurovascular recovery process. Multiexposure speckle imaging (MESI) provides a quantitatively accurate technique for chronically measuring the postocclusion CBF perfusion of the infarct and peri-infarct regions in rodent stroke models, while multiphoton microscopy offers direct visualization of the microvascular structure. In this paper, we present imaging outcomes extending 35 days after photo-thrombotic occlusion, tracking the progression of the vasculature throughout this period. We compare MESI flow estimates within the unresolvable parenchyma with subsurface microvascular volume fractions taken with two-photon microscopy in the same regions to assess how the vascular density influences the surface-integrated MESI flow values. The MESI flow measurements and volume fractions are shown to have high correlations (r=0.90) within areas of recovering vasculature in the peri-infarct region. We also observe vascular reorientation occurring within the microvascular structure throughout the 35-day postocclusion period. With the combination of a chronic mouse model and relatively noninvasive optical imaging techniques, we present an imaging protocol for monitoring long-term vascular progression after photo-thrombotic occlusion with the potential to test the efficacy of rehabilitation and pharmacological therapies.
  • |Animals [MESH]
  • |Brain/*blood supply/pathology [MESH]
  • |Cerebrovascular Circulation [MESH]
  • |Male [MESH]
  • |Mice [MESH]
  • |Microscopy, Fluorescence, Multiphoton [MESH]
  • |Microvessels/*pathology [MESH]
  • |Optical Imaging [MESH]


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