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2015 ; 35
(6
): 933-42
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Chronic monitoring of vascular progression after ischemic stroke using
multiexposure speckle imaging and two-photon fluorescence microscopy
#MMPMID25712498
Schrandt CJ
; Kazmi SM
; Jones TA
; Dunn AK
J Cereb Blood Flow Metab
2015[Jun]; 35
(6
): 933-42
PMID25712498
show ga
Monitoring the progression of the vascular structure and cerebral blood flow
(CBF) after brain injury is vital to understand the neurovascular recovery
process. Multiexposure speckle imaging (MESI) provides a quantitatively accurate
technique for chronically measuring the postocclusion CBF perfusion of the
infarct and peri-infarct regions in rodent stroke models, while multiphoton
microscopy offers direct visualization of the microvascular structure. In this
paper, we present imaging outcomes extending 35 days after photo-thrombotic
occlusion, tracking the progression of the vasculature throughout this period. We
compare MESI flow estimates within the unresolvable parenchyma with subsurface
microvascular volume fractions taken with two-photon microscopy in the same
regions to assess how the vascular density influences the surface-integrated MESI
flow values. The MESI flow measurements and volume fractions are shown to have
high correlations (r=0.90) within areas of recovering vasculature in the
peri-infarct region. We also observe vascular reorientation occurring within the
microvascular structure throughout the 35-day postocclusion period. With the
combination of a chronic mouse model and relatively noninvasive optical imaging
techniques, we present an imaging protocol for monitoring long-term vascular
progression after photo-thrombotic occlusion with the potential to test the
efficacy of rehabilitation and pharmacological therapies.